| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 101 | Bacillus wiedmannii | TGACGAAACGACTAGCGGA | TTCAGCTGGGTGAGATGCT | 58.75 | 58.62 | 250 | 73 |
100.00%
|
100.00%
|
| Primer 102 | Bacillus wiedmannii | ACCGAGCGCAAATGGAAC | TCACCCGTCGTGTGGAAA | 58.74 | 58.78 | 154 | 73 |
100.00%
|
100.00%
|
| Primer 103 | Bacillus wiedmannii | TCCGACAGTTGCAACGCT | GTATGCACCAACGACCCCA | 59.89 | 60.00 | 232 | 73 |
100.00%
|
100.00%
|
| Primer 104 | Bacillus wiedmannii | TCCGACAGTTGCAACGCT | ATGCACCAACGACCCCAAA | 59.89 | 60.15 | 230 | 73 |
100.00%
|
100.00%
|
| Primer 105 | Bacillus wiedmannii | TCCGACAGTTGCAACGCT | TGCACCAACGACCCCAAAA | 59.89 | 60.38 | 229 | 73 |
100.00%
|
100.00%
|
| Primer 106 | Bacillus wiedmannii | TTACAGCCGACACGCGAA | TCAACTGCTGCTGCTGCT | 59.66 | 59.89 | 155 | 73 |
100.00%
|
100.00%
|
| Primer 107 | Bacillus wiedmannii | CGAAGATGCTGGTTGGGGT | CCAGTCTCCATGTGGCAACT | 60.00 | 59.96 | 253 | 73 |
100.00%
|
100.00%
|
| Primer 108 | Bacillus wiedmannii | CAGCGGAAGTAGTTGGTGGT | CAAAACCGTCTGCTTTCGCA | 59.97 | 59.97 | 210 | 73 |
100.00%
|
100.00%
|
| Primer 109 | Bacillus wiedmannii | GCCGAAAGCACTAGGTGGT | TGAGAGACGCCAGCAACA | 60.00 | 58.86 | 225 | 73 |
100.00%
|
100.00%
|
| Primer 110 | Bacillus wiedmannii | GCCGAAAGCACTAGGTGGT | GTCATGAGAGACGCCAGCA | 60.00 | 59.78 | 229 | 73 |
100.00%
|
100.00%
|