| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Bifidobacterium longum | CCTGCAAGAGACCCATGCA | AATCGGTATCGCCTTGGGC | 60.00 | 60.23 | 203 | 127 |
100.00%
|
60.00%
|
| Primer 2 | Bifidobacterium longum | TGACCCTGCAAGAGACCCA | AATCGGTATCGCCTTGGGC | 60.46 | 60.23 | 207 | 124 |
100.00%
|
60.00%
|
| Primer 3 | Bifidobacterium longum | TTCAGCCGAAGAGCCATGG | TTGCGCACACACGTTTGG | 60.08 | 59.90 | 155 | 122 |
100.00%
|
80.00%
|
| Primer 4 | Bifidobacterium longum | ACCTGCCACACCATCAGTC | AATCGGTATCGCCTTGGGC | 59.62 | 60.23 | 168 | 121 |
100.00%
|
20.00%
|
| Primer 5 | Bifidobacterium longum | ACCTGCCACACCATCAGT | AATCGGTATCGCCTTGGGC | 58.42 | 60.23 | 168 | 121 |
100.00%
|
20.00%
|
| Primer 6 | Bifidobacterium longum | TTCAGCCGAAGAGCCATGG | TCAGGCATTGCTCACGCA | 60.08 | 59.97 | 182 | 116 |
100.00%
|
80.00%
|
| Primer 7 | Bifidobacterium longum | AACGAACACCGACTTCCCC | ATTGCGTTTGCTCGTGCC | 59.93 | 59.74 | 211 | 116 |
100.00%
|
60.00%
|
| Primer 8 | Bifidobacterium longum | GTGCATTGCAACACGCCA | TTCTGCCGACTTTGCCGT | 59.97 | 59.89 | 171 | 112 |
100.00%
|
60.00%
|
| Primer 9 | Bifidobacterium longum | AAAGCCCATGCGGACGAA | TTCTGCCGACTTTGCCGT | 59.97 | 59.89 | 205 | 112 |
100.00%
|
60.00%
|
| Primer 10 | Bifidobacterium longum | TGCAACACGCCAAGGTGA | TTCTGCCGACTTTGCCGT | 60.12 | 59.89 | 165 | 112 |
100.00%
|
60.00%
|