| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 61 | Bordetella avium | ACCGATCCTGTGCGCAAA | CTGGCCATTGGTTTGCACC | 59.97 | 60.00 | 249 | 126 |
100.00%
|
100.00%
|
| Primer 62 | Bordetella avium | ACCGATCCTGTGCGCAAA | TGGCCATTGGTTTGCACC | 59.97 | 58.85 | 248 | 126 |
100.00%
|
100.00%
|
| Primer 63 | Bordetella avium | ACCGATCCTGTGCGCAAA | GGACTGGCCATTGGTTTGC | 59.97 | 59.71 | 252 | 126 |
100.00%
|
100.00%
|
| Primer 64 | Bordetella avium | CCCTGCCCAAGCTCAAAGA | TGCCATCCAGCTTTGCCA | 59.92 | 59.88 | 258 | 125 |
100.00%
|
100.00%
|
| Primer 65 | Bordetella avium | AAGGCTTCCGAATTGCGC | TCACGCCTGCAATGCTGA | 59.12 | 59.97 | 166 | 125 |
100.00%
|
100.00%
|
| Primer 66 | Bordetella avium | AGCGACGCCAGCTTTGAA | TGAGACTTCGAGAGTGCGC | 60.28 | 59.79 | 272 | 125 |
100.00%
|
100.00%
|
| Primer 67 | Bordetella avium | AGCGACGCCAGCTTTGAA | GCCTGGCCATCCTTGAAGA | 60.28 | 59.70 | 236 | 125 |
100.00%
|
100.00%
|
| Primer 68 | Bordetella avium | TTCCCGAACGCTTGCAGA | TGCCGTCGATGTTCAGCA | 59.58 | 59.66 | 189 | 125 |
100.00%
|
100.00%
|
| Primer 69 | Bordetella avium | TTTGCTCACCGATGCCCA | AGCCTTGGTTGCGCTCTT | 59.57 | 59.89 | 257 | 125 |
100.00%
|
100.00%
|
| Primer 70 | Bordetella avium | TTGCTCACCGATGCCCAT | AGCCTTGGTTGCGCTCTT | 59.32 | 59.89 | 256 | 125 |
100.00%
|
100.00%
|