| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Bordetella pertussis | ACCCCATCTGCGCAATCA | AGTTGCAGGCGTTCCTTGT | 59.32 | 60.15 | 235 | 38 |
100.00%
|
100.00%
|
| Primer 42 | Bordetella pertussis | TGGAGTTCGGTTTGCGCA | GCCAGCACTCTCGCGATAT | 60.20 | 60.01 | 160 | 37 |
100.00%
|
100.00%
|
| Primer 43 | Bordetella pertussis | ACCACCCACCGCATTCTT | GCACGCATTTGCTCGTTG | 59.16 | 58.56 | 269 | 37 |
100.00%
|
100.00%
|
| Primer 44 | Bordetella pertussis | CGTTCAAGCCCTCGACCTT | AATCCGACAGGCAGGCAA | 60.00 | 59.24 | 252 | 37 |
100.00%
|
100.00%
|
| Primer 45 | Bordetella pertussis | CGTTCAAGCCCTCGACCTT | AAATCCGACAGGCAGGCA | 60.00 | 59.24 | 253 | 37 |
100.00%
|
100.00%
|
| Primer 46 | Bordetella pertussis | CGTTCAAGCCCTCGACCTT | ATCCGACAGGCAGGCAAT | 60.00 | 59.00 | 251 | 37 |
100.00%
|
100.00%
|
| Primer 47 | Bordetella pertussis | ACGGCAGTTCCAGCAGAA | CGGCTGAATTCCTCGACGT | 59.17 | 60.15 | 162 | 36 |
100.00%
|
100.00%
|
| Primer 48 | Bordetella pertussis | TGCACAAAATCGCCAGCG | TGCTCCCACATGTTGCCT | 59.75 | 59.15 | 165 | 34 |
100.00%
|
100.00%
|
| Primer 49 | Bordetella pertussis | AAGACCACCCACCGCATT | GCACGCATTTGCTCGTTG | 59.16 | 58.56 | 272 | 24 |
100.00%
|
100.00%
|