| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 491 | Brachyspira murdochii | GGCTGGATGGTTTACAGAGGT | AGACTGCTGAACTACGCCTT | 59.72 | 59.03 | 162 | 68 |
100.00%
|
100.00%
|
| Primer 492 | Brachyspira murdochii | ACGGCAGAGGAAGACTTGAG | AGCCAGTAAGCACCGTCA | 59.39 | 58.53 | 198 | 68 |
100.00%
|
100.00%
|
| Primer 493 | Brachyspira murdochii | TGCAAGCAGAAGAGAAGCTGA | AGTCCGCTGCTGTCTTTATCA | 59.93 | 59.45 | 279 | 68 |
100.00%
|
100.00%
|
| Primer 494 | Brachyspira murdochii | TGACGGCACTACATCAACAGA | AAGGTGCTTTGCCTCCTCT | 59.38 | 58.84 | 200 | 68 |
100.00%
|
100.00%
|
| Primer 495 | Brachyspira murdochii | TGCCAACCGACCTGATGA | GCAAGCGGACATACATGAGC | 58.52 | 59.69 | 249 | 68 |
100.00%
|
100.00%
|
| Primer 496 | Brachyspira murdochii | ATGGTGCTGGGGAGCTAGT | AGCAGCAAGACCAGCATCA | 60.31 | 59.62 | 188 | 68 |
100.00%
|
100.00%
|
| Primer 497 | Brachyspira murdochii | ACTTCAGGAGAGCCTCTCACT | CTCTGCCGTCCAAAACCTCA | 59.92 | 60.25 | 211 | 68 |
100.00%
|
100.00%
|
| Primer 498 | Brachyspira murdochii | GCCTAAGAAGAAGCATGCCC | GCGTAACTCCGTAGCCTTCA | 58.97 | 59.83 | 215 | 68 |
100.00%
|
100.00%
|
| Primer 499 | Brachyspira murdochii | GGCCTAAGAAGAAGCATGCC | GCGTAACTCCGTAGCCTTCA | 58.97 | 59.83 | 216 | 68 |
100.00%
|
100.00%
|
| Primer 500 | Brachyspira murdochii | GTGCTAGGGGAGTGCTTCA | ACCCCATACCCCTGAAGTATC | 59.02 | 58.30 | 268 | 68 |
100.00%
|
100.00%
|