| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 61 | Brachyspira murdochii | AAGTGCCCAGGCCAATGT | AGGAGCAGACAAAGGAGCA | 59.47 | 58.54 | 202 | 95 |
100.00%
|
100.00%
|
| Primer 62 | Brachyspira murdochii | GAGGGTGTTAATCCTGTGGCA | CCCCAATGAGGTGCACAGT | 60.00 | 59.92 | 212 | 95 |
100.00%
|
100.00%
|
| Primer 63 | Brachyspira murdochii | AGGGTGTTAATCCTGTGGCA | CCCCAATGAGGTGCACAGT | 58.93 | 59.92 | 211 | 95 |
100.00%
|
100.00%
|
| Primer 64 | Brachyspira murdochii | ACCGTTTCTTGCTTGGGC | GCAAACACGACCGCACAA | 58.56 | 59.59 | 162 | 94 |
100.00%
|
100.00%
|
| Primer 65 | Brachyspira murdochii | ACCGTTTCTTGCTTGGGC | CACGACCGCACAAAGCAA | 58.56 | 59.29 | 157 | 94 |
100.00%
|
100.00%
|
| Primer 66 | Brachyspira murdochii | AAGGCGTACGTTTGGGAGG | AACCATCGCCAACAGGGTC | 60.00 | 60.30 | 180 | 94 |
100.00%
|
100.00%
|
| Primer 67 | Brachyspira murdochii | GCGGAGAATCGCGTGAGAT | TCTTCCCAAACTTCCTCCGG | 60.30 | 59.31 | 284 | 94 |
100.00%
|
100.00%
|
| Primer 68 | Brachyspira murdochii | TCCTGCCGATGTAGATGTTGG | GCCTCCTGCAATTGAGCTTC | 59.86 | 59.54 | 240 | 94 |
100.00%
|
100.00%
|
| Primer 69 | Brachyspira murdochii | TGCTAGGGGAGTGCTTCAC | ACCCCATACCCCTGAAGTATC | 59.02 | 58.30 | 267 | 94 |
100.00%
|
100.00%
|
| Primer 70 | Brachyspira murdochii | TCCTGTGGCAGTGGATTCAAA | CCCCAATGAGGTGCACAGT | 59.86 | 59.92 | 201 | 94 |
100.00%
|
100.00%
|