| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Brachyspira pilosicoli | GCGTTCCTTGCCCAAAACC | TCGTTTCCCTGTCCCATAGC | 60.30 | 59.46 | 271 | 145 |
100.00%
|
100.00%
|
| Primer 2 | Brachyspira pilosicoli | GCGTTCCTTGCCCAAAACC | ATCGTTTCCCTGTCCCATAGC | 60.30 | 59.86 | 272 | 145 |
100.00%
|
100.00%
|
| Primer 3 | Brachyspira pilosicoli | TTGCTTGCCCTGCTTCTCC | CAGCTTCTCACTCCGTCTGG | 60.60 | 60.11 | 214 | 120 |
100.00%
|
100.00%
|
| Primer 4 | Brachyspira pilosicoli | TGGAGAACAGAGCGGAGAAC | ACCACCTTTGTCAACCAACAT | 59.40 | 58.25 | 187 | 118 |
100.00%
|
100.00%
|
| Primer 5 | Brachyspira pilosicoli | GCGGTTACATGGGAGAGGAT | CCTCTTCAACGCTGTCAAGC | 59.24 | 59.49 | 183 | 110 |
100.00%
|
100.00%
|
| Primer 6 | Brachyspira pilosicoli | TGGGCAAAACAGTATGGCAT | GGCTCGGCAAACTTAATACCC | 58.06 | 59.33 | 236 | 109 |
100.00%
|
100.00%
|
| Primer 7 | Brachyspira pilosicoli | GCGGTTACATGGGAGAGGA | CCTCTTCAACGCTGTCAAGC | 58.79 | 59.49 | 183 | 109 |
100.00%
|
100.00%
|
| Primer 8 | Brachyspira pilosicoli | TGCTGAAGAATGGGCAATGG | GGTCTCACCAAAGCACAAGC | 58.81 | 59.69 | 246 | 107 |
100.00%
|
50.00%
|
| Primer 9 | Brachyspira pilosicoli | AGGTGGCAAGGCTTCTCAC | TCCCCATCAAGCCTCACAC | 59.93 | 59.31 | 157 | 107 |
100.00%
|
50.00%
|
| Primer 10 | Brachyspira pilosicoli | AGACGCTGATGCCATGCA | TGCGGTACACGTCATCATTGT | 59.73 | 60.34 | 197 | 107 |
100.00%
|
100.00%
|