| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 301 | Bradyrhizobium diazoefficiens | TTCGTGATCGCTGGCCAA | GCGAAATGCCGTGATCGTC | 59.65 | 60.01 | 202 | 50 |
100.00%
|
100.00%
|
| Primer 302 | Bradyrhizobium diazoefficiens | TCGCATTCCGTTCGAGCA | TCTTCACGCCAAGACGGTC | 59.74 | 60.01 | 275 | 50 |
100.00%
|
100.00%
|
| Primer 303 | Bradyrhizobium diazoefficiens | TTGATCGTGGATCCCGAGG | CGGAAACTCCAGCACGACT | 58.88 | 60.01 | 168 | 50 |
100.00%
|
100.00%
|
| Primer 304 | Bradyrhizobium diazoefficiens | TCCACCATTGCCTTCGACC | AATCGGTCAGCATGCCCT | 60.00 | 59.00 | 292 | 50 |
100.00%
|
100.00%
|
| Primer 305 | Bradyrhizobium diazoefficiens | TCACCTTTCAGACCTGCGG | ACGGCTTGAAGCTCGCAT | 59.63 | 60.05 | 276 | 50 |
100.00%
|
100.00%
|
| Primer 306 | Bradyrhizobium diazoefficiens | TTTTGCGTTGCTGCCCTG | CCGGAGAGCTGCTTTGGAT | 59.58 | 59.78 | 159 | 49 |
100.00%
|
100.00%
|
| Primer 307 | Bradyrhizobium diazoefficiens | CAAGTTCGTCTTCGTGCGC | TCAGCACCGCAACGATCA | 60.15 | 59.66 | 194 | 49 |
100.00%
|
100.00%
|
| Primer 308 | Bradyrhizobium diazoefficiens | AAGTTCGTCTTCGTGCGC | TCAGCACCGCAACGATCA | 58.77 | 59.66 | 193 | 49 |
100.00%
|
100.00%
|
| Primer 309 | Bradyrhizobium diazoefficiens | TCCTGGTGGACGAGGAACA | AGGCCAGATGATAGCGCAC | 60.15 | 59.93 | 297 | 49 |
100.00%
|
100.00%
|
| Primer 310 | Bradyrhizobium diazoefficiens | TCCTGGTGGACGAGGAACA | GGCCAGATGATAGCGCACA | 60.15 | 60.23 | 296 | 49 |
100.00%
|
100.00%
|