| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 61 | Bradyrhizobium diazoefficiens | ACATCGCCATGAACCGCT | CTTCGAATTGCGGATGGCC | 59.73 | 59.64 | 279 | 64 |
100.00%
|
100.00%
|
| Primer 62 | Bradyrhizobium diazoefficiens | ACATCGCCATGAACCGCT | CCTTCGAATTGCGGATGGC | 59.73 | 59.64 | 280 | 64 |
100.00%
|
100.00%
|
| Primer 63 | Bradyrhizobium diazoefficiens | ACATCGCCATGAACCGCT | GCCTTCGAATTGCGGATGG | 59.73 | 59.64 | 281 | 64 |
100.00%
|
100.00%
|
| Primer 64 | Bradyrhizobium diazoefficiens | ACATCGCCATGAACCGCT | TTCGAATTGCGGATGGCC | 59.73 | 58.49 | 278 | 64 |
100.00%
|
100.00%
|
| Primer 65 | Bradyrhizobium diazoefficiens | TGCGCAACTGCTCGTTCT | GCGTAGGCGAACCAGATCA | 60.28 | 59.86 | 220 | 63 |
100.00%
|
100.00%
|
| Primer 66 | Bradyrhizobium diazoefficiens | TGCGCAACTGCTCGTTCT | AGGCGAACCAGATCACGAC | 60.28 | 59.78 | 216 | 63 |
100.00%
|
100.00%
|
| Primer 67 | Bradyrhizobium diazoefficiens | TGCGCAACTGCTCGTTCT | ACGTCGGTGAAGAAGTGCC | 60.28 | 60.30 | 172 | 63 |
100.00%
|
100.00%
|
| Primer 68 | Bradyrhizobium diazoefficiens | AGACCAGCGCAAAAGCCA | GCGAGGCCACGAACTTCAT | 60.20 | 60.74 | 186 | 63 |
100.00%
|
100.00%
|
| Primer 69 | Bradyrhizobium diazoefficiens | TTCCGGTGCTGCAATCCA | CAGCACGATGGCGATGTTG | 59.57 | 59.94 | 209 | 63 |
100.00%
|
100.00%
|
| Primer 70 | Bradyrhizobium diazoefficiens | AGCGGATCATCGAGTTGGC | ACAGGCCTTCACGTCATGG | 60.23 | 60.00 | 164 | 63 |
100.00%
|
100.00%
|