| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 61 | Brevibacillus brevis | TTGGGACGCATGGAGGAT | AGGAGAGAACGGAGGCGAT | 58.26 | 60.08 | 244 | 121 |
100.00%
|
100.00%
|
| Primer 62 | Brevibacillus brevis | ACGGGTTGCCAAGCTTGA | TCCATGATGGTCACGTTCGG | 59.81 | 60.11 | 177 | 120 |
100.00%
|
100.00%
|
| Primer 63 | Brevibacillus brevis | CACGACGGGATTCGGACAA | AATTGCTGCCACCCCACT | 60.08 | 59.47 | 226 | 120 |
100.00%
|
100.00%
|
| Primer 64 | Brevibacillus brevis | ACGACGGGATTCGGACAA | AATTGCTGCCACCCCACT | 58.63 | 59.47 | 225 | 120 |
100.00%
|
100.00%
|
| Primer 65 | Brevibacillus brevis | AAGGCGAAACGATTGCGG | TGCATGCCTTCCTGCACA | 59.13 | 59.88 | 181 | 120 |
100.00%
|
100.00%
|
| Primer 66 | Brevibacillus brevis | GAAGGCGAAACGATTGCGG | TGCATGCCTTCCTGCACA | 60.23 | 59.88 | 182 | 120 |
100.00%
|
100.00%
|
| Primer 67 | Brevibacillus brevis | CGTCACGCGAGCATTCAAG | GTTCGATGGCAGGCAGGAT | 59.94 | 60.15 | 163 | 120 |
100.00%
|
100.00%
|
| Primer 68 | Brevibacillus brevis | TCGATTGGGACGCATGGA | AGGAGAGAACGGAGGCGAT | 58.70 | 60.08 | 248 | 120 |
100.00%
|
100.00%
|
| Primer 69 | Brevibacillus brevis | GATTGGGACGCATGGAGGA | AGGAGAGAACGGAGGCGAT | 59.47 | 60.08 | 246 | 120 |
100.00%
|
100.00%
|
| Primer 70 | Brevibacillus brevis | ATTGGGACGCATGGAGGA | AGGAGAGAACGGAGGCGAT | 58.26 | 60.08 | 245 | 120 |
100.00%
|
100.00%
|