| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 31 | Brevibacillus massiliensis | TTTGGTGCTGCCGACGAT | CGACATTTCGCGTCAGGGA | 59.97 | 60.45 | 233 | 81 |
100.00%
|
100.00%
|
| Primer 32 | Brevibacillus massiliensis | AAATGCTGCGCAATGCCC | TGCCTAAAAGCGCTTGGC | 60.13 | 59.04 | 195 | 81 |
100.00%
|
100.00%
|
| Primer 33 | Brevibacillus massiliensis | CGGCAAACGCGTTCACAT | TTTCGCTCCTGCACGCAT | 59.75 | 60.36 | 157 | 81 |
100.00%
|
100.00%
|
| Primer 34 | Brevibacillus massiliensis | CGGCAAACGCGTTCACAT | AGGGTGTGAAGCGGAACAG | 59.75 | 59.93 | 249 | 81 |
100.00%
|
100.00%
|
| Primer 35 | Brevibacillus massiliensis | CGGCAAACGCGTTCACAT | ATCCCTTTCGCTCCTGCAC | 59.75 | 60.08 | 162 | 81 |
100.00%
|
100.00%
|
| Primer 36 | Brevibacillus massiliensis | TGCACAAGCGGTTGAAGC | GGAGTTCGGCATCTTCTTTGG | 59.28 | 59.26 | 161 | 81 |
100.00%
|
100.00%
|
| Primer 37 | Brevibacillus massiliensis | TTGCACAAGCGGTTGAAGC | GGAGTTCGGCATCTTCTTTGG | 59.93 | 59.26 | 162 | 81 |
100.00%
|
100.00%
|
| Primer 38 | Brevibacillus massiliensis | AGATCCCGACTGTCGCCAT | AGTCCTCTCCACGAGCTGT | 60.76 | 59.92 | 163 | 80 |
100.00%
|
100.00%
|
| Primer 39 | Brevibacillus massiliensis | TGGTGCATGCTTCGCTGT | TTGGCCAAGACGTAGCTGG | 60.28 | 60.00 | 226 | 80 |
100.00%
|
100.00%
|
| Primer 40 | Brevibacillus massiliensis | GTGCCAAAACGCCAAGCA | CGATAAGCTGACGTTGCCG | 59.89 | 59.37 | 152 | 80 |
100.00%
|
100.00%
|