| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 481 | Chlorobium limicola | TGCACTGCAGGAACTCGA | ACAAGCAGGCCAGCATCA | 58.86 | 59.56 | 165 | 76 |
100.00%
|
100.00%
|
| Primer 482 | Chlorobium limicola | TGCTGCTGCACATCCCAT | CGGTCGCAATCGGGATAGT | 59.64 | 59.64 | 168 | 76 |
100.00%
|
100.00%
|
| Primer 483 | Chlorobium limicola | TGACGCCATCGAGCAGTT | TGGATGAGGCACCCGTTT | 59.34 | 58.83 | 161 | 76 |
100.00%
|
100.00%
|
| Primer 484 | Chlorobium limicola | TGTGCCATCATGCGCTCA | GCTGCGCAAAACTGCCAT | 60.05 | 60.05 | 261 | 76 |
100.00%
|
100.00%
|
| Primer 485 | Chlorobium limicola | TGTGCCATCATGCGCTCA | ACAAACCGCTGCGCAAAA | 60.05 | 59.51 | 268 | 76 |
100.00%
|
100.00%
|
| Primer 486 | Chlorobium limicola | TGTGCCATCATGCGCTCA | AACAAACCGCTGCGCAAA | 60.05 | 59.51 | 269 | 76 |
100.00%
|
100.00%
|
| Primer 487 | Chlorobium limicola | ACGCCTATGGTCGACGTTG | AACGCAACTCTCCGCTCA | 60.15 | 59.27 | 256 | 76 |
100.00%
|
100.00%
|
| Primer 488 | Chlorobium limicola | GCGAAGATGATGGCCTCGA | ACGGCTGCGTTTTCCTCA | 59.93 | 59.89 | 233 | 76 |
100.00%
|
100.00%
|
| Primer 489 | Chlorobium limicola | AACGGCAAGGACGAAACGA | ACGGCTGCGTTTTCCTCA | 60.23 | 59.89 | 296 | 76 |
100.00%
|
100.00%
|
| Primer 490 | Chlorobium limicola | TCGTCGGGTTGCCAATGA | TGCTGCCGTCAATGCGAT | 59.26 | 60.44 | 203 | 76 |
100.00%
|
100.00%
|