| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Citrobacter sedlakii | TTGGATGCTTTGCGCTGC | ACGTCCGTCATGATCGGTG | 59.74 | 59.86 | 164 | 114 |
100.00%
|
100.00%
|
| Primer 2 | Citrobacter sedlakii | ACGTGCTGAGCGAAATGC | TTTGCGCTCAATGCCAGC | 59.14 | 59.74 | 223 | 114 |
100.00%
|
100.00%
|
| Primer 3 | Citrobacter sedlakii | TTCGATCAGCCGACGCAT | TCCACGCACGCTGAACAT | 59.51 | 59.97 | 173 | 111 |
100.00%
|
100.00%
|
| Primer 4 | Citrobacter sedlakii | ACAATCCGGACGACGTTCC | AGCCGTCTTTGCAGCCAT | 60.08 | 59.97 | 270 | 111 |
100.00%
|
100.00%
|
| Primer 5 | Citrobacter sedlakii | TGCCTGAAGGGTCGGAGAT | TGGCGTTGGCGATCATGT | 60.31 | 60.05 | 288 | 110 |
100.00%
|
100.00%
|
| Primer 6 | Citrobacter sedlakii | TTCAGTCGATGCCACGCA | ACTGCCTGCGCATTCTGT | 59.66 | 59.97 | 276 | 107 |
100.00%
|
100.00%
|
| Primer 7 | Citrobacter sedlakii | AGCGATTGCCGATGAGCA | TGAAGGTGTTGTGCGGTGT | 59.81 | 60.08 | 204 | 107 |
100.00%
|
100.00%
|
| Primer 8 | Citrobacter sedlakii | AGCGATTGCCGATGAGCA | GCGGATGAAGGTGTTGTGC | 59.81 | 59.79 | 209 | 107 |
100.00%
|
100.00%
|
| Primer 9 | Citrobacter sedlakii | AGCGTTTCCGAACTGGCA | CGTTCTCGCTAACAGGGCA | 59.89 | 60.08 | 258 | 107 |
100.00%
|
100.00%
|
| Primer 10 | Citrobacter sedlakii | AGCGTTTCCGAACTGGCA | GCAAACTCGGCAAGCTGAC | 59.89 | 60.08 | 185 | 107 |
100.00%
|
100.00%
|