| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Clostridium butyricum | CAACAGTGGCTGCATCAGG | CGCTATTACATGGGCAACAGC | 59.12 | 60.00 | 173 | 166 |
100.00%
|
50.00%
|
| Primer 2 | Clostridium butyricum | CCAACAGTGGCTGCATCAG | CGCTATTACATGGGCAACAGC | 59.12 | 60.00 | 174 | 166 |
100.00%
|
50.00%
|
| Primer 3 | Clostridium butyricum | AGTGGCTGCATCAGGTATGG | CGCTATTACATGGGCAACAGC | 59.82 | 60.00 | 169 | 166 |
100.00%
|
50.00%
|
| Primer 4 | Clostridium butyricum | CAGTTCGCTGCGCTTATGG | CCTCCACACCATCATAGCTGG | 59.65 | 60.20 | 272 | 159 |
100.00%
|
50.00%
|
| Primer 5 | Clostridium butyricum | TGAACAAGCAAAGCTGGCAG | TGCGCATGAGCAATCTGC | 59.61 | 59.20 | 182 | 158 |
100.00%
|
50.00%
|
| Primer 6 | Clostridium butyricum | ATGAACAAGCAAAGCTGGCAG | TGCGCATGAGCAATCTGC | 60.00 | 59.20 | 183 | 158 |
100.00%
|
50.00%
|
| Primer 7 | Clostridium butyricum | GCGGTTGCTTTTGGTAGTCC | TGTGCTTTTGTTGGTGACTCC | 59.76 | 59.25 | 233 | 152 |
100.00%
|
50.00%
|
| Primer 8 | Clostridium butyricum | TCGCTGCGCTTATGGTCAG | CCTCCACACCATCATAGCTGG | 60.52 | 60.20 | 268 | 151 |
100.00%
|
50.00%
|
| Primer 9 | Clostridium butyricum | GCATGTGCAACTACCGCTG | TGCCGTAACAGATTTCCTTGG | 59.86 | 58.57 | 228 | 145 |
100.00%
|
50.00%
|
| Primer 10 | Clostridium butyricum | GCTTGATGAACCAGCAGCTG | CCCCTTTCCAAGATACGCTTC | 59.83 | 58.71 | 238 | 143 |
100.00%
|
50.00%
|