| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1211 | Clostridium butyricum | ATGGGAGGGTGGGTCAATT | TCCCAATCCCCTGACCTTTTC | 58.18 | 59.64 | 252 | 52 |
100.00%
|
50.00%
|
| Primer 1212 | Clostridium butyricum | CAGGTTCCGGATGATTCGGT | GTCCTCTGCTCCAACCGTT | 59.82 | 59.63 | 174 | 52 |
100.00%
|
50.00%
|
| Primer 1213 | Clostridium butyricum | AGGTGTTTTGGGGACAGGA | TTAGCGACCCTCTGCTCGT | 58.36 | 60.68 | 269 | 52 |
100.00%
|
50.00%
|
| Primer 1214 | Clostridium butyricum | ACTCAAAGAGTTGCCATTGCT | AGTGCAACATTTACGCCTGTT | 58.41 | 59.05 | 299 | 52 |
100.00%
|
50.00%
|
| Primer 1215 | Clostridium butyricum | TGTTGGTGCTGATGGAATTGG | CCCCTACTGGAGGCTCTTCA | 59.11 | 60.33 | 275 | 52 |
100.00%
|
50.00%
|
| Primer 1216 | Clostridium butyricum | CTGCTTGTTGGTTTTGGCGT | CGCCAAGTACTCCTCCAAGT | 60.18 | 59.39 | 151 | 52 |
100.00%
|
50.00%
|
| Primer 1217 | Clostridium butyricum | CTGCTTGTTGGTTTTGGCGT | ACGCCAAGTACTCCTCCAAG | 60.18 | 59.39 | 152 | 52 |
100.00%
|
50.00%
|
| Primer 1218 | Clostridium butyricum | ACGGGATTGCAGATTACGGA | TGTGCAACCACTGGTAAACCT | 59.17 | 60.06 | 208 | 52 |
100.00%
|
50.00%
|
| Primer 1219 | Clostridium butyricum | TGGATATGAAGGAGAGGCGAG | TCAGTTTCTGCCTCATTCCCT | 58.76 | 59.01 | 269 | 52 |
100.00%
|
50.00%
|
| Primer 1220 | Clostridium butyricum | GGATATGAAGGAGAGGCGAGA | TCAGTTTCTGCCTCATTCCCT | 58.48 | 59.01 | 268 | 52 |
100.00%
|
50.00%
|