| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1301 | Clostridium butyricum | AGGTGTGTGTGGTGAAGGC | AGGTTGTCCTACGCCTACTT | 60.15 | 58.07 | 280 | 45 |
100.00%
|
50.00%
|
| Primer 1302 | Clostridium butyricum | TGAATGCATGGGGAGACAGA | GATGCACATACAACAGCGCT | 58.71 | 59.27 | 172 | 45 |
100.00%
|
50.00%
|
| Primer 1303 | Clostridium butyricum | TGAATGCATGGGGAGACAGA | ATGCACATACAACAGCGCT | 58.71 | 58.14 | 171 | 45 |
100.00%
|
50.00%
|
| Primer 1304 | Clostridium butyricum | AGCTTACCCTTACCATAGCGG | ACTGTCCAGCGTTCACAGA | 59.31 | 58.88 | 282 | 45 |
100.00%
|
50.00%
|
| Primer 1305 | Clostridium butyricum | CAGGTGCTGTGCTGGTAAC | CCGCTTGGCATACCTCCTT | 58.75 | 59.78 | 217 | 45 |
100.00%
|
50.00%
|
| Primer 1306 | Clostridium butyricum | TCAAGGGCTTCCAAAGGGG | TGTTTGCTTGCTTCTAAGGCA | 59.54 | 58.69 | 168 | 45 |
100.00%
|
50.00%
|
| Primer 1307 | Clostridium butyricum | AAGGGCATTGATGTGAAGCTT | GCTTTCCAAATGCAACTCCCC | 58.47 | 60.34 | 298 | 45 |
100.00%
|
50.00%
|
| Primer 1308 | Clostridium butyricum | AAGGGCATTGATGTGAAGCTT | GGCTTTCCAAATGCAACTCCC | 58.47 | 60.34 | 299 | 45 |
100.00%
|
50.00%
|
| Primer 1309 | Clostridium butyricum | ATTTGTTGCTGCCAGCGG | AACTGCATGTACAACGTCCTT | 59.35 | 58.15 | 160 | 45 |
100.00%
|
50.00%
|
| Primer 1310 | Clostridium butyricum | AATTTGTTGCTGCCAGCGG | AACTGCATGTACAACGTCCTT | 60.01 | 58.15 | 161 | 45 |
100.00%
|
50.00%
|