| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Clostridium butyricum | TGCTTGATGAACCAGCAGC | CCCCTTTCCAAGATACGCTTC | 58.74 | 58.71 | 239 | 143 |
100.00%
|
50.00%
|
| Primer 12 | Clostridium butyricum | TTGCTTGATGAACCAGCAGC | CCCCTTTCCAAGATACGCTTC | 59.40 | 58.71 | 240 | 143 |
100.00%
|
50.00%
|
| Primer 13 | Clostridium butyricum | ATTGCTTGATGAACCAGCAGC | CCCCTTTCCAAGATACGCTTC | 59.80 | 58.71 | 241 | 143 |
100.00%
|
50.00%
|
| Primer 14 | Clostridium butyricum | TGCTTGATGAACCAGCAGCTG | CCCCTTTCCAAGATACGCTTC | 61.49 | 58.71 | 239 | 143 |
100.00%
|
50.00%
|
| Primer 15 | Clostridium butyricum | CTGCAGGTGTTGTAGGAACTG | ACCCACAACAATCCGGTCC | 58.85 | 59.93 | 291 | 142 |
100.00%
|
50.00%
|
| Primer 16 | Clostridium butyricum | GCTGCAAAGGAAACAGGAGC | CTGGCTTTCCGCTTTCACC | 60.04 | 59.42 | 154 | 136 |
100.00%
|
50.00%
|
| Primer 17 | Clostridium butyricum | GAACAAGCAAAGCTGGCAGT | TGCGCATGAGCAATCTGC | 59.61 | 59.20 | 181 | 133 |
100.00%
|
50.00%
|
| Primer 18 | Clostridium butyricum | AACAAGCAAAGCTGGCAGT | TGCGCATGAGCAATCTGC | 58.49 | 59.20 | 180 | 133 |
100.00%
|
50.00%
|
| Primer 19 | Clostridium butyricum | AGGAAGTGGTTTGGGGAGT | TGCCCTCCAACTCTGTCAC | 58.04 | 59.25 | 237 | 133 |
100.00%
|
50.00%
|
| Primer 20 | Clostridium butyricum | TCAACAGTTCCACCCCTCC | AGGTGCAATTGATTCCGTGAT | 58.85 | 58.27 | 297 | 131 |
100.00%
|
50.00%
|