| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 51 | Clostridium butyricum | AGGAAGTGGTTTGGGGAGT | ATGCCCTCCAACTCTGTCAC | 58.04 | 59.67 | 238 | 123 |
100.00%
|
50.00%
|
| Primer 52 | Clostridium butyricum | AGGAAGTGGTTTGGGGAGT | TCCGCTTACAATATGCCCTCC | 58.04 | 59.93 | 250 | 123 |
100.00%
|
50.00%
|
| Primer 53 | Clostridium butyricum | AGGAACTGGAGCTGCAGT | ACCGCTGATAGCCACTTGG | 58.10 | 59.78 | 163 | 123 |
100.00%
|
50.00%
|
| Primer 54 | Clostridium butyricum | TCAGGAAATGCAGGGCAGG | AGCAACATCTTCAGGCTTTCC | 60.00 | 58.83 | 222 | 122 |
100.00%
|
50.00%
|
| Primer 55 | Clostridium butyricum | TGGCTCAGATACCTAGGGGA | TCACCAAGTCCATCAGGTTGT | 58.76 | 59.23 | 163 | 120 |
100.00%
|
50.00%
|
| Primer 56 | Clostridium butyricum | TCTCCAGGAGTAAAGCTTGGA | ACTACCGCACCAGCTCCAA | 58.10 | 61.21 | 260 | 120 |
100.00%
|
50.00%
|
| Primer 57 | Clostridium butyricum | GCAGCTACTCCCCTTGCAA | TCCGCTTGTAGAAAGGCTTGA | 60.00 | 59.65 | 213 | 120 |
100.00%
|
50.00%
|
| Primer 58 | Clostridium butyricum | GCAGCTACTCCCCTTGCAA | ACCGGTACAAGTTGTGTGACT | 60.00 | 59.52 | 245 | 120 |
100.00%
|
50.00%
|
| Primer 59 | Clostridium butyricum | GCAGCTACTCCCCTTGCAA | CCGCTTGTAGAAAGGCTTGA | 60.00 | 58.19 | 212 | 120 |
100.00%
|
50.00%
|
| Primer 60 | Clostridium butyricum | GCAGCTACTCCCCTTGCAA | TCCGCTTGTAGAAAGGCTTG | 60.00 | 58.19 | 213 | 120 |
100.00%
|
50.00%
|