| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 61 | Clostridium butyricum | GCAGCTACTCCCCTTGCAA | AGTTCCGCTTGTAGAAAGGC | 60.00 | 58.19 | 216 | 120 |
100.00%
|
50.00%
|
| Primer 62 | Clostridium butyricum | GCAGCTACTCCCCTTGCAA | ACCGGTACAAGTTGTGTGAC | 60.00 | 58.06 | 245 | 120 |
100.00%
|
50.00%
|
| Primer 63 | Clostridium butyricum | GCAGCTACTCCCCTTGCAA | TTCCGCTTGTAGAAAGGCTTG | 60.00 | 58.85 | 214 | 120 |
100.00%
|
50.00%
|
| Primer 64 | Clostridium butyricum | GGTCTTTGGGAGCTAATGGGA | CCGCTTGCACATTCACTGT | 59.44 | 59.05 | 214 | 120 |
100.00%
|
50.00%
|
| Primer 65 | Clostridium butyricum | GGTCTTTGGGAGCTAATGGGA | ACCGCTTGCACATTCACTG | 59.44 | 59.05 | 215 | 120 |
100.00%
|
50.00%
|
| Primer 66 | Clostridium butyricum | GCCACAAGTATGCCGAATGC | TGCTATGGTACGTCCGCTC | 60.25 | 59.27 | 262 | 120 |
100.00%
|
50.00%
|
| Primer 67 | Clostridium butyricum | TCATGGCCTGCAACTGTGA | AGGCAAGTGGAACGGCAA | 59.54 | 59.81 | 166 | 120 |
100.00%
|
50.00%
|
| Primer 68 | Clostridium butyricum | TCATGGCCTGCAACTGTGAT | AGGCAAGTGGAACGGCAA | 59.96 | 59.81 | 166 | 120 |
100.00%
|
50.00%
|
| Primer 69 | Clostridium butyricum | TTCATGGCCTGCAACTGTGA | AGGCAAGTGGAACGGCAA | 60.18 | 59.81 | 167 | 120 |
100.00%
|
50.00%
|
| Primer 70 | Clostridium butyricum | ACAAGACTTGGGCTTGGACC | TGGAATCCACATGACACCACT | 60.18 | 59.30 | 278 | 118 |
100.00%
|
50.00%
|