| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Clostridium cadaveris | AAGGACGGGAAGCAGAAGG | TCATCGGTAAGCGGAACAGG | 59.32 | 59.83 | 158 | 121 |
100.00%
|
100.00%
|
| Primer 2 | Clostridium cadaveris | GCAATGCAGTGCTACCAGG | TCCAAATCCTCCGGACACC | 59.19 | 59.01 | 203 | 119 |
100.00%
|
100.00%
|
| Primer 3 | Clostridium cadaveris | TGCAGGAAGTTGACAATGCTG | TAGCGCTGCCAAGAGAACC | 59.39 | 60.08 | 240 | 114 |
100.00%
|
100.00%
|
| Primer 4 | Clostridium cadaveris | ACCAGGTACAGCGGATTTGG | ACCAAGCTCGCTTTGTGC | 60.04 | 58.97 | 220 | 110 |
100.00%
|
100.00%
|
| Primer 5 | Clostridium cadaveris | GCTGATGAACCAACAGGAGC | AACATTTGCGCTGCCACC | 59.19 | 59.66 | 267 | 108 |
100.00%
|
100.00%
|
| Primer 6 | Clostridium cadaveris | TTGGCTGATGAACCAACAGG | AACATTTGCGCTGCCACC | 58.38 | 59.66 | 270 | 108 |
100.00%
|
100.00%
|
| Primer 7 | Clostridium cadaveris | GCACGTGTAAAGTCCCAGC | CCGGAGTTTCTGCCAAAAGC | 59.13 | 60.04 | 184 | 108 |
100.00%
|
100.00%
|
| Primer 8 | Clostridium cadaveris | TATGGGTTGGAGCTGCAGC | TGAACTTACCGCACCTAAAGC | 60.08 | 58.58 | 266 | 108 |
100.00%
|
100.00%
|
| Primer 9 | Clostridium cadaveris | TTATGGGTTGGAGCTGCAGC | TGAACTTACCGCACCTAAAGC | 60.68 | 58.58 | 267 | 108 |
100.00%
|
100.00%
|
| Primer 10 | Clostridium cadaveris | ACGAGAGTATCCACGTGACTC | ACCCCACCTCGTCATAAGG | 58.99 | 58.40 | 195 | 107 |
100.00%
|
100.00%
|