| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Clostridium carboxidivorans | TGCTATGGCTGCTTATGAAGC | TGTGCAGGACTATGGCCAG | 58.78 | 59.40 | 207 | 121 |
100.00%
|
100.00%
|
| Primer 2 | Clostridium carboxidivorans | TGCTATGGCTGCTTATGAAGC | ATGTGCAGGACTATGGCCAG | 58.78 | 59.82 | 208 | 121 |
100.00%
|
100.00%
|
| Primer 3 | Clostridium carboxidivorans | CGGCAACAGGCTTTATCTTGG | AAGCCACCACCAGGAGTTG | 59.87 | 59.85 | 192 | 117 |
100.00%
|
100.00%
|
| Primer 4 | Clostridium carboxidivorans | AAGATGCCTGCTATGAAGTGG | TCTGATCATCATCAAGCGCTG | 58.07 | 58.52 | 192 | 112 |
100.00%
|
100.00%
|
| Primer 5 | Clostridium carboxidivorans | AGCTCGCGTTTCATTTGCTC | GGTGTATGCTCCACAAATGGC | 59.83 | 59.87 | 237 | 107 |
100.00%
|
100.00%
|
| Primer 6 | Clostridium carboxidivorans | TCGCGTTTCATTTGCTCCTG | GGTGTATGCTCCACAAATGGC | 59.48 | 59.87 | 234 | 107 |
100.00%
|
100.00%
|
| Primer 7 | Clostridium carboxidivorans | GCTCGCGTTTCATTTGCTC | GGTGTATGCTCCACAAATGGC | 58.37 | 59.87 | 236 | 107 |
100.00%
|
100.00%
|
| Primer 8 | Clostridium carboxidivorans | TGCAGCTATAAGGGCAGGAG | AGGGGTCCACTATCCAGCA | 59.24 | 59.60 | 224 | 102 |
100.00%
|
100.00%
|
| Primer 9 | Clostridium carboxidivorans | TGCAGCTATAAGGGCAGGA | AGGGGTCCACTATCCAGCA | 58.07 | 59.60 | 224 | 102 |
100.00%
|
100.00%
|
| Primer 10 | Clostridium carboxidivorans | GATGCAGCTATAAGGGCAGGA | AGGGGTCCACTATCCAGCA | 59.65 | 59.60 | 226 | 102 |
100.00%
|
100.00%
|