| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 251 | Clostridium septicum | TGGGAATGAGTACTCTGCTGG | CTCTTCTTCAGTTTCCCCACC | 59.17 | 58.22 | 259 | 102 |
100.00%
|
100.00%
|
| Primer 252 | Clostridium septicum | CCCGAAACACCTACCCTTCA | TCTCCTTTGAAGTGAGCAGCA | 59.31 | 59.58 | 170 | 102 |
100.00%
|
100.00%
|
| Primer 253 | Clostridium septicum | ACGGGAGGAAGGAGAATGTG | GGTTAAGCTCTTCGGCACAAC | 59.09 | 59.80 | 260 | 101 |
100.00%
|
100.00%
|
| Primer 254 | Clostridium septicum | AGCACTGAAGCTGGAAATGC | CGCCAGGTTCACTTGCATC | 59.11 | 59.50 | 190 | 101 |
100.00%
|
100.00%
|
| Primer 255 | Clostridium septicum | CTTGGTGGAGGAAATGGGGA | TCTGCTGTCCATGGAGCAC | 59.30 | 59.70 | 266 | 100 |
100.00%
|
100.00%
|
| Primer 256 | Clostridium septicum | TTGGTGGAGGAAATGGGGA | TCTGCTGTCCATGGAGCAC | 58.11 | 59.70 | 265 | 100 |
100.00%
|
100.00%
|
| Primer 257 | Clostridium septicum | TGGCGTTGGTGAAGTTGGA | AACTGGTGGAAGCCCATCC | 59.78 | 59.62 | 226 | 100 |
100.00%
|
100.00%
|
| Primer 258 | Clostridium septicum | AGGCTTCTGGGGAACTTGC | TACACAACCTGCAGCTCCC | 59.92 | 59.63 | 176 | 100 |
100.00%
|
100.00%
|
| Primer 259 | Clostridium septicum | TGCTGGATTTGCTGGGTGT | ACACCCCATGAAAGTCCCC | 59.85 | 59.23 | 238 | 100 |
100.00%
|
100.00%
|
| Primer 260 | Clostridium septicum | AGGTGCGTTGGCTGCTAT | GCCGTTCTCTCTTCGCCAT | 59.33 | 60.15 | 186 | 99 |
100.00%
|
100.00%
|