| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 221 | Clostridium tepidum | GCGCTACCTCTAATACAGCCA | AGGATGCCATTCCCATCTTGT | 59.66 | 59.43 | 173 | 91 |
100.00%
|
100.00%
|
| Primer 222 | Clostridium tepidum | GCGCTACCTCTAATACAGCCA | GGTGTTTAGGATGCCATTCCC | 59.66 | 58.97 | 180 | 91 |
100.00%
|
100.00%
|
| Primer 223 | Clostridium tepidum | GCGCTACCTCTAATACAGCCA | GGGTGTTTAGGATGCCATTCC | 59.66 | 58.97 | 181 | 91 |
100.00%
|
100.00%
|
| Primer 224 | Clostridium tepidum | GCGCTACCTCTAATACAGCCA | GGTTAGGGTGTTTAGGATGCC | 59.66 | 58.35 | 186 | 91 |
100.00%
|
100.00%
|
| Primer 225 | Clostridium tepidum | CCAGAGTGGGTTAAAACACCA | GCGCTTCCTTTGTTGAGCA | 58.06 | 59.35 | 259 | 91 |
100.00%
|
100.00%
|
| Primer 226 | Clostridium tepidum | CCAGAGTGGGTTAAAACACCA | AGCGCTTCCTTTGTTGAGC | 58.06 | 59.05 | 260 | 91 |
100.00%
|
100.00%
|
| Primer 227 | Clostridium tepidum | CCAGAGTGGGTTAAAACACCA | AAGCGCTTCCTTTGTTGAGC | 58.06 | 59.69 | 261 | 91 |
100.00%
|
100.00%
|
| Primer 228 | Clostridium tepidum | CCAGAGTGGGTTAAAACACCA | GCGCTTCCTTTGTTGAGCAG | 58.06 | 60.39 | 259 | 91 |
100.00%
|
100.00%
|
| Primer 229 | Clostridium tepidum | CCAGAGTGGGTTAAAACACCA | AGCGCTTCCTTTGTTGAGCA | 58.06 | 60.82 | 260 | 91 |
100.00%
|
100.00%
|
| Primer 230 | Clostridium tepidum | GCAGATAGGGTTGTGTTTCCA | TCTTCCGCAACTGGTGATACA | 58.21 | 59.38 | 239 | 91 |
100.00%
|
100.00%
|