| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 271 | Clostridium tepidum | TCATGGTGGCTAGGGCAA | AGCATTCCAAGACCAGGCC | 58.17 | 60.00 | 166 | 86 |
100.00%
|
100.00%
|
| Primer 272 | Clostridium tepidum | TGGTTCTAACGGTTGTGGCAA | GACTCTGTCTATAGCGGTGGA | 60.41 | 58.43 | 217 | 86 |
100.00%
|
100.00%
|
| Primer 273 | Clostridium tepidum | AGTGGTCCTACTCGTCACAA | TTGAAACTAGCGCAGGAGC | 58.01 | 58.16 | 169 | 86 |
100.00%
|
100.00%
|
| Primer 274 | Clostridium tepidum | AGTGGTCCTACTCGTCACAA | ATTGAAACTAGCGCAGGAGC | 58.01 | 58.63 | 170 | 86 |
100.00%
|
100.00%
|
| Primer 275 | Clostridium tepidum | GCGGTCACAGAGTTGGGAT | AGCGAATCCATCCCATCAGAT | 59.70 | 59.02 | 247 | 85 |
100.00%
|
100.00%
|
| Primer 276 | Clostridium tepidum | TTGCAGATGAAGTTGGCGAT | TCTGCCATTGGACACCCAG | 58.17 | 59.62 | 169 | 85 |
100.00%
|
100.00%
|
| Primer 277 | Clostridium tepidum | GGAGGACCAAATGCAGATGC | AACCGCTATTCCTGCTGGAC | 59.26 | 60.11 | 177 | 85 |
100.00%
|
100.00%
|
| Primer 278 | Clostridium tepidum | AGCATTGCAGGCTAGAGACA | GCATCCCCTCCATCATGGT | 59.09 | 59.15 | 168 | 84 |
100.00%
|
100.00%
|
| Primer 279 | Clostridium tepidum | AGCATTGCAGGCTAGAGACAA | GCATCCCCTCCATCATGGT | 59.72 | 59.15 | 168 | 84 |
100.00%
|
100.00%
|
| Primer 280 | Clostridium tepidum | AAGCATTGCAGGCTAGAGACA | GCATCCCCTCCATCATGGT | 59.72 | 59.15 | 169 | 84 |
100.00%
|
100.00%
|