| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Clostridium tetani | AACCGCAGAATCAAAGGGAC | GCTACCCATACCAGGAATGGC | 58.47 | 60.55 | 163 | 141 |
100.00%
|
100.00%
|
| Primer 22 | Clostridium tetani | TGGCAACGGGATATTCAGGA | AGAACAAGGTGCAGCGTGT | 58.79 | 60.15 | 239 | 140 |
100.00%
|
100.00%
|
| Primer 23 | Clostridium tetani | AACCGCAGAATCAAAGGGAC | GCAAAGCTACCCATACCAGGA | 58.47 | 59.79 | 168 | 140 |
100.00%
|
100.00%
|
| Primer 24 | Clostridium tetani | ATTCCCCTCAAGCAGTGGC | TGCTTGCTATAAGGGCAGGA | 60.00 | 58.79 | 207 | 140 |
100.00%
|
100.00%
|
| Primer 25 | Clostridium tetani | ATTCCCCTCAAGCAGTGGC | ATGCTTGCTATAAGGGCAGGA | 60.00 | 59.22 | 208 | 140 |
100.00%
|
100.00%
|
| Primer 26 | Clostridium tetani | TTGGGCAACTTGGTGTGG | TGTAGGAAATGCTTGCACTCC | 58.05 | 58.56 | 220 | 138 |
100.00%
|
100.00%
|
| Primer 27 | Clostridium tetani | TGGCGTTGAAGAGGATAGACT | AGCTCCTCCAACCTCTACTTC | 58.54 | 58.54 | 151 | 138 |
100.00%
|
100.00%
|
| Primer 28 | Clostridium tetani | ACTCAACCAGGACTCAGTGG | GCAAGGAAGCGCATATTCACC | 58.95 | 60.27 | 240 | 138 |
100.00%
|
100.00%
|
| Primer 29 | Clostridium tetani | AGGTCAAGGGGATTGCACT | TCTTCATGAGGATGGGTAGCA | 58.52 | 58.24 | 153 | 138 |
100.00%
|
100.00%
|
| Primer 30 | Clostridium tetani | TGTAGGTCAAGGGGATTGCAC | TCTTCATGAGGATGGGTAGCA | 60.00 | 58.24 | 156 | 138 |
100.00%
|
100.00%
|