| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 81 | Comamonas kerstersii | TGCCAGCTGCCTATGTCTC | AAAGCAGCGCAGACGTCT | 59.48 | 59.97 | 167 | 127 |
100.00%
|
100.00%
|
| Primer 82 | Comamonas kerstersii | TCTGCCAGCTGCCTATGTC | AAAGCAGCGCAGACGTCT | 59.48 | 59.97 | 169 | 127 |
100.00%
|
100.00%
|
| Primer 83 | Comamonas kerstersii | TCCGCACACACACTTGCA | ATGAGCACCCAGTCACAGC | 60.12 | 60.00 | 282 | 127 |
100.00%
|
100.00%
|
| Primer 84 | Comamonas kerstersii | TCCGCACACACACTTGCA | TGCAATCCACAGCTGCAC | 60.12 | 58.64 | 190 | 127 |
100.00%
|
100.00%
|
| Primer 85 | Comamonas kerstersii | ACATGACGCAGCAGCCAT | GCAAAGGCAATACCGCTGG | 60.05 | 59.86 | 237 | 127 |
100.00%
|
100.00%
|
| Primer 86 | Comamonas kerstersii | ACATGACGCAGCAGCCAT | CGCAAAGGCAATACCGCTG | 60.05 | 60.23 | 238 | 127 |
100.00%
|
100.00%
|
| Primer 87 | Comamonas kerstersii | ACACCACCAACAACCGCA | ATCCAGTTCCTGCCACAGC | 60.04 | 60.00 | 203 | 127 |
100.00%
|
100.00%
|
| Primer 88 | Comamonas kerstersii | ACACCACCAACAACCGCA | GCTGTGCTGACCAGGTGAT | 60.04 | 60.00 | 220 | 127 |
100.00%
|
100.00%
|
| Primer 89 | Comamonas kerstersii | ACACCACCAACAACCGCA | TGTGCTGACCAGGTGATCC | 60.04 | 59.32 | 218 | 127 |
100.00%
|
100.00%
|
| Primer 90 | Comamonas kerstersii | ACAAGTGCCTGACGCTGT | GCTTGCGCTGTGGAAACA | 59.49 | 59.28 | 282 | 126 |
100.00%
|
100.00%
|