| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Comamonas testosteroni | ATGCACGCATGGCTTTGC | AAGACAGGCCACATGCTGG | 60.13 | 60.30 | 264 | 157 |
100.00%
|
50.00%
|
| Primer 2 | Comamonas testosteroni | TCTCTGGTCATGCTGCTGG | AGCAGGAACTTGGCCATGG | 59.40 | 60.30 | 224 | 145 |
100.00%
|
50.00%
|
| Primer 3 | Comamonas testosteroni | AAGCAGCCCATCGAGTTCC | TCGAAGTTCAGCGTGGCA | 60.08 | 59.58 | 167 | 138 |
100.00%
|
50.00%
|
| Primer 4 | Comamonas testosteroni | ATGCACGCATGGCTTTGC | CCCGAAAGACAGGCCACAT | 60.13 | 60.00 | 269 | 138 |
100.00%
|
50.00%
|
| Primer 5 | Comamonas testosteroni | TTTGCGCACCACATTGGC | TGCTGGTAGCTGTTGGCA | 59.97 | 59.16 | 234 | 138 |
100.00%
|
50.00%
|
| Primer 6 | Comamonas testosteroni | TTTGCGCACCACATTGGC | GCGATCCTTGATCTGGGCA | 59.97 | 59.85 | 268 | 138 |
100.00%
|
50.00%
|
| Primer 7 | Comamonas testosteroni | TGTCGAAGATCGCGAGGA | AGCGCAAGACATCCTGCA | 58.10 | 59.65 | 239 | 138 |
100.00%
|
50.00%
|
| Primer 8 | Comamonas testosteroni | TGTCGAAGATCGCGAGGATG | AGCGCAAGACATCCTGCA | 59.97 | 59.65 | 239 | 138 |
100.00%
|
50.00%
|
| Primer 9 | Comamonas testosteroni | GTCGAAGATCGCGAGGATGT | AGCGCAAGACATCCTGCA | 59.97 | 59.65 | 238 | 138 |
100.00%
|
50.00%
|
| Primer 10 | Comamonas testosteroni | AGTGGCAGCTGATTCCCAC | TGGCGTCGGCAAAGTCAT | 60.00 | 59.97 | 279 | 138 |
100.00%
|
50.00%
|