| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive | 
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Cupriavidus metallidurans | TGTGTCCGTCCAACGATGG | ACCAAGATCGTCGGCATCC | 60.01 | 59.86 | 222 | 115 | 
                                                 
                                                        100.00%
                                                     
                                                 | 
                                            
                                                 
                                                        100.00%
                                                     
                                                 | 
                                        
| Primer 2 | Cupriavidus metallidurans | AGTCGAAGGCATGTCCTGC | TCAGCTTGCCGACTTGACC | 60.08 | 60.30 | 187 | 108 | 
                                                 
                                                        100.00%
                                                     
                                                 | 
                                            
                                                 
                                                        100.00%
                                                     
                                                 | 
                                        
| Primer 3 | Cupriavidus metallidurans | TTGCGACGTGAAGGCCAT | CGACCAGATCGCTCTGCAT | 59.97 | 59.93 | 214 | 102 | 
                                                 
                                                        100.00%
                                                     
                                                 | 
                                            
                                                 
                                                        100.00%
                                                     
                                                 | 
                                        
| Primer 4 | Cupriavidus metallidurans | TTGCGACGTGAAGGCCAT | ACCAGATCGCTCTGCATGG | 59.97 | 59.85 | 212 | 102 | 
                                                 
                                                        100.00%
                                                     
                                                 | 
                                            
                                                 
                                                        100.00%
                                                     
                                                 | 
                                        
| Primer 5 | Cupriavidus metallidurans | ATCCAGACAGGCCAGTTGC | AAGGCAGGTTGGACACAGG | 60.00 | 59.85 | 165 | 101 | 
                                                 
                                                        100.00%
                                                     
                                                 | 
                                            
                                                 
                                                        100.00%
                                                     
                                                 | 
                                        
| Primer 6 | Cupriavidus metallidurans | TGCGCTGATTCGACCGAT | TCCACGCAATCACGCTGT | 59.51 | 59.97 | 185 | 101 | 
                                                 
                                                        100.00%
                                                     
                                                 | 
                                            
                                                 
                                                        100.00%
                                                     
                                                 | 
                                        
| Primer 7 | Cupriavidus metallidurans | ATGCGCTGATTCGACCGAT | TCCACGCAATCACGCTGT | 59.93 | 59.97 | 186 | 101 | 
                                                 
                                                        100.00%
                                                     
                                                 | 
                                            
                                                 
                                                        100.00%
                                                     
                                                 | 
                                        
| Primer 8 | Cupriavidus metallidurans | AAGCGTCTGGGCAAGTACC | TGACGGCAAGCATCGTGT | 60.00 | 59.97 | 217 | 101 | 
                                                 
                                                        100.00%
                                                     
                                                 | 
                                            
                                                 
                                                        100.00%
                                                     
                                                 | 
                                        
| Primer 9 | Cupriavidus metallidurans | TGAAGCCAACTCGGTGGTG | ATGCGGACACGACGTTGT | 60.23 | 59.97 | 176 | 101 | 
                                                 
                                                        100.00%
                                                     
                                                 | 
                                            
                                                 
                                                        100.00%
                                                     
                                                 | 
                                        
| Primer 10 | Cupriavidus metallidurans | GCAATGCGAACGTGCTGT | GGGTGGCATAGGCGTTCAT | 59.75 | 60.15 | 159 | 101 | 
                                                 
                                                        100.00%
                                                     
                                                 | 
                                            
                                                 
                                                        100.00%
                                                     
                                                 |