| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Desulfitobacterium hafniense | ATGAGGGGTGGTTTGGCAG | TAGCTGAGCCGTTGCATCC | 59.92 | 60.15 | 182 | 131 |
100.00%
|
100.00%
|
| Primer 2 | Desulfitobacterium hafniense | AACTCAGGGCTGCCAAAGG | CCGTGTATTGTCCCGTTGC | 60.23 | 59.21 | 170 | 124 |
100.00%
|
100.00%
|
| Primer 3 | Desulfitobacterium hafniense | GCCGTTCTGCCAGAAAAGC | CAGTTGCTGCGCTTACGTG | 60.08 | 60.15 | 181 | 123 |
100.00%
|
100.00%
|
| Primer 4 | Desulfitobacterium hafniense | ACTGGGGATTGTTGCCTGG | AAAACGCTGTTGGGCTGG | 59.92 | 58.87 | 273 | 120 |
100.00%
|
100.00%
|
| Primer 5 | Desulfitobacterium hafniense | GCCGTTCTGCCAGAAAAGC | AGTTGCTGCGCTTACGTG | 60.08 | 58.76 | 180 | 117 |
100.00%
|
100.00%
|
| Primer 6 | Desulfitobacterium hafniense | ACCCTTTGTCTGGCGATGG | AAAGCCTCCGTATCGCACC | 60.00 | 60.15 | 152 | 107 |
100.00%
|
100.00%
|
| Primer 7 | Desulfitobacterium hafniense | TCTCATGAGGATGCCCTGG | AGCGGTGTGACAAGGTTCC | 58.46 | 60.23 | 166 | 101 |
100.00%
|
100.00%
|
| Primer 8 | Desulfitobacterium hafniense | TGGCTACTTCTTCCGCAGC | TTATCGCACCTGAGCCCAC | 60.08 | 59.78 | 215 | 100 |
100.00%
|
100.00%
|
| Primer 9 | Desulfitobacterium hafniense | TCTCGGCAGGACGAATGTG | ACGCTGCTGTAACGCTGT | 59.78 | 59.97 | 227 | 98 |
100.00%
|
100.00%
|
| Primer 10 | Desulfitobacterium hafniense | AATCGGGTTCCCACAGCTG | TGGGCAGCTTAGGCAGTTC | 60.00 | 60.00 | 196 | 98 |
100.00%
|
100.00%
|