| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Enterococcus asini | ACATCGTCGAGTTGCGCA | ACCCGAGCGATACAAACCC | 60.05 | 59.78 | 247 | 138 |
100.00%
|
100.00%
|
| Primer 12 | Enterococcus asini | ACATCGTCGAGTTGCGCA | AGGCACTGGTCGGTTCATC | 60.05 | 59.70 | 297 | 138 |
100.00%
|
100.00%
|
| Primer 13 | Enterococcus asini | TGGAACGCCAAATCCGCA | ACAGTCGTTTCGTCCGGAC | 60.28 | 60.01 | 153 | 138 |
100.00%
|
100.00%
|
| Primer 14 | Enterococcus asini | TGGAACGCCAAATCCGCA | AGACCGCTTCTTCGCTGT | 60.28 | 58.95 | 186 | 138 |
100.00%
|
100.00%
|
| Primer 15 | Enterococcus asini | TGGAACGCCAAATCCGCA | GACAGTCGTTTCGTCCGGA | 60.28 | 59.72 | 154 | 138 |
100.00%
|
100.00%
|
| Primer 16 | Enterococcus asini | AGGACACGCATTCGGCAT | TACTCGTCCCCATGGCTGT | 59.73 | 60.31 | 158 | 137 |
100.00%
|
100.00%
|
| Primer 17 | Enterococcus asini | ACATCGTCGAGTTGCGCA | CCAACACCCGAGCGATACA | 60.05 | 59.78 | 252 | 137 |
100.00%
|
100.00%
|
| Primer 18 | Enterococcus asini | TGGAACGCCAAATCCGCA | CAGTCGTTTCGTCCGGACA | 60.28 | 60.01 | 152 | 137 |
100.00%
|
100.00%
|
| Primer 19 | Enterococcus asini | ACGCCACCGGATTTGCAT | ACCCGATCCACGCGAAAA | 60.36 | 59.66 | 209 | 137 |
100.00%
|
100.00%
|
| Primer 20 | Enterococcus asini | ATCACCTGGCTGGGCAAA | TTGCTAACCACGCTGCCA | 59.15 | 59.89 | 238 | 135 |
100.00%
|
100.00%
|