| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Enterococcus avium | ACGTCACACCGCAAACCA | CTCCCTTCAGTGACACGCA | 60.13 | 59.63 | 205 | 135 |
100.00%
|
100.00%
|
| Primer 12 | Enterococcus avium | ACGTCACACCGCAAACCA | TCCCTTCAGTGACACGCA | 60.13 | 58.46 | 204 | 135 |
100.00%
|
100.00%
|
| Primer 13 | Enterococcus avium | ACGTCACACCGCAAACCA | CTCCCTTCAGTGACACGCAT | 60.13 | 60.04 | 205 | 135 |
100.00%
|
100.00%
|
| Primer 14 | Enterococcus avium | ACGCGTCTGCAAAGCTTG | TGTCCGATTGTCGCACCA | 59.37 | 59.26 | 209 | 135 |
100.00%
|
100.00%
|
| Primer 15 | Enterococcus avium | AAAGACGCAGGCAGAGCA | AGCGCACTCGTTGGTTCA | 59.57 | 59.89 | 162 | 132 |
100.00%
|
100.00%
|
| Primer 16 | Enterococcus avium | CAAGCAAAGACGCAGGCA | AGCGCACTCGTTGGTTCA | 58.97 | 59.89 | 167 | 132 |
100.00%
|
100.00%
|
| Primer 17 | Enterococcus avium | CAAGCAAAGACGCAGGCAG | AGCGCACTCGTTGGTTCA | 60.08 | 59.89 | 167 | 132 |
100.00%
|
100.00%
|
| Primer 18 | Enterococcus avium | GAGCAAGCAAAGACGCAGG | AGCGCACTCGTTGGTTCA | 59.79 | 59.89 | 170 | 132 |
100.00%
|
100.00%
|
| Primer 19 | Enterococcus avium | TGCACGAGCCTGAGTTAGC | TCGTCGAACAGCGCTTGA | 60.08 | 59.67 | 181 | 132 |
100.00%
|
100.00%
|
| Primer 20 | Enterococcus avium | GAAAGAGCGAGCACGAAGC | AATGCTCCAACCGCAAGC | 59.58 | 59.04 | 211 | 132 |
100.00%
|
100.00%
|