| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 31 | Escherichia albertii | ATTGGCGAACGGTTGCTG | TGTTCCAGCCATTGCCCA | 59.05 | 59.47 | 278 | 102 |
100.00%
|
50.00%
|
| Primer 32 | Escherichia albertii | ACGATGTCGCTGAAGCCA | TTTTGCTCTCGCGAGGCA | 59.34 | 59.97 | 223 | 102 |
100.00%
|
50.00%
|
| Primer 33 | Escherichia albertii | TCGCACACGGATGACCAT | TCGGTAATTCGCACGCCA | 59.02 | 59.74 | 165 | 102 |
100.00%
|
50.00%
|
| Primer 34 | Escherichia albertii | AAGCTGACCGTCGAACTGG | TCGGTAATTCGCACGCCA | 60.01 | 59.74 | 185 | 102 |
100.00%
|
50.00%
|
| Primer 35 | Escherichia albertii | TGAGGCGATGTTCCAGCA | TCGGTAATTCGCACGCCA | 58.93 | 59.74 | 267 | 102 |
100.00%
|
50.00%
|
| Primer 36 | Escherichia albertii | AGGCGATGTTCCAGCAGT | TCGGTAATTCGCACGCCA | 58.93 | 59.74 | 265 | 102 |
100.00%
|
50.00%
|
| Primer 37 | Escherichia albertii | GAGGCGATGTTCCAGCAGT | TCGGTAATTCGCACGCCA | 60.08 | 59.74 | 266 | 102 |
100.00%
|
50.00%
|
| Primer 38 | Escherichia albertii | TGAGGCGATGTTCCAGCAG | TCGGTAATTCGCACGCCA | 60.08 | 59.74 | 267 | 102 |
100.00%
|
50.00%
|
| Primer 39 | Escherichia albertii | CTGAGGCGATGTTCCAGCA | TCGGTAATTCGCACGCCA | 60.08 | 59.74 | 268 | 102 |
100.00%
|
50.00%
|
| Primer 40 | Escherichia albertii | AGCGCAATTAAGCCTCGGT | TGCCAGTTGATGCAGCGT | 60.08 | 60.28 | 172 | 101 |
100.00%
|
50.00%
|