| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Escherichia fergusonii | CCAATCGTGATGGCCTGGA | CAACGCGTCGCACCAAAA | 59.78 | 59.98 | 205 | 101 |
100.00%
|
50.00%
|
| Primer 12 | Escherichia fergusonii | AAACCGTCTCGCCATCCA | TGAGCCAGCACAATCGCA | 58.93 | 59.97 | 205 | 99 |
100.00%
|
50.00%
|
| Primer 13 | Escherichia fergusonii | AGGTCGCAGCGGTATTACG | TTTCGCCACGGCTTCACT | 59.93 | 59.89 | 254 | 96 |
100.00%
|
50.00%
|
| Primer 14 | Escherichia fergusonii | ACCAGTTCAGCAGCTGCA | CGCTGGCGACTTCCTTTTG | 59.49 | 59.79 | 196 | 95 |
100.00%
|
50.00%
|
| Primer 15 | Escherichia fergusonii | ATCGTGTGGAGTTGCGCA | ACCAAACTGCGGACGTGT | 59.97 | 59.81 | 235 | 95 |
100.00%
|
50.00%
|
| Primer 16 | Escherichia fergusonii | AGCAGCATTTCGCCAGGA | ACCACTGCTTCGATCGCA | 59.65 | 59.34 | 153 | 95 |
100.00%
|
50.00%
|
| Primer 17 | Escherichia fergusonii | AACGTTGCCAGACTCGCA | TTTTGCCACGCCAGAAGC | 59.89 | 59.27 | 151 | 95 |
100.00%
|
50.00%
|
| Primer 18 | Escherichia fergusonii | AGCAAGGCGAAATTGTGGC | TGTTTCCAGGCATCGGCA | 59.71 | 59.57 | 232 | 95 |
100.00%
|
50.00%
|
| Primer 19 | Escherichia fergusonii | TGCTGGTAACCTTCACGCC | TGCAGCAGCGCAAAGACT | 60.30 | 60.59 | 264 | 95 |
100.00%
|
50.00%
|
| Primer 20 | Escherichia fergusonii | ACCGTGTCCTCAATTGGCC | TGCAGCAGCGCAAAGACT | 60.30 | 60.59 | 209 | 95 |
100.00%
|
50.00%
|