| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Exiguobacterium sibiricum | ACGTGTCGCAGGTGTTGT | ATTGTCCCTCTTCCGCTGC | 59.81 | 60.08 | 269 | 148 |
100.00%
|
100.00%
|
| Primer 2 | Exiguobacterium sibiricum | ACGGTGCCAAGGAAGTCAC | ACAATTTGGCGAGCGTGC | 60.23 | 59.74 | 273 | 146 |
100.00%
|
100.00%
|
| Primer 3 | Exiguobacterium sibiricum | CCCATGCGATGAACGGGAT | AACTGCTCCGCTTGCGAT | 60.23 | 60.05 | 260 | 141 |
100.00%
|
100.00%
|
| Primer 4 | Exiguobacterium sibiricum | GCGGACAGTGTCTTTCGGA | TCGTGTTGCTGCGACAGT | 60.01 | 59.89 | 237 | 140 |
100.00%
|
100.00%
|
| Primer 5 | Exiguobacterium sibiricum | CCAAACGTTCCTTCGCAGC | ATAGAGTGGAGCACGCGTG | 60.08 | 59.86 | 155 | 140 |
100.00%
|
100.00%
|
| Primer 6 | Exiguobacterium sibiricum | GCCAAACGTTCCTTCGCAG | ATAGAGTGGAGCACGCGTG | 60.08 | 59.86 | 156 | 140 |
100.00%
|
100.00%
|
| Primer 7 | Exiguobacterium sibiricum | TCACGGGATTCCTCGGTTC | CCAGGTTTGCAAAGACGCC | 59.10 | 60.01 | 168 | 138 |
100.00%
|
100.00%
|
| Primer 8 | Exiguobacterium sibiricum | TTGTCGGAGCAGGAACACC | ACCGGAATGACTTGCGCA | 59.93 | 59.97 | 253 | 138 |
100.00%
|
100.00%
|
| Primer 9 | Exiguobacterium sibiricum | AGCAAGCGGTGTCGTCAT | AGCGTGACAGTTCCTGCA | 59.66 | 59.18 | 237 | 138 |
100.00%
|
100.00%
|
| Primer 10 | Exiguobacterium sibiricum | ATATTCGGCAGCACGGCA | GCAAACAGCGGATGCAGT | 59.81 | 59.04 | 160 | 138 |
100.00%
|
100.00%
|