| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Ezakiella coagulans | AGTGCGAAGGCTCAAAAGG | TCACCGTAAACGCCTTTACC | 58.37 | 58.21 | 157 | 138 |
100.00%
|
100.00%
|
| Primer 12 | Ezakiella coagulans | TGCAATTGGACAGGCGACT | ACGAGCTTTGTGCCGTAGT | 59.93 | 59.63 | 192 | 138 |
100.00%
|
100.00%
|
| Primer 13 | Ezakiella coagulans | GCAATTGGACAGGCGACT | ACGAGCTTTGTGCCGTAGT | 58.01 | 59.63 | 191 | 138 |
100.00%
|
100.00%
|
| Primer 14 | Ezakiella coagulans | GGCGATGGATGATTTAGCCG | CCCTTTGATTCCGGAGTAAGC | 59.20 | 58.71 | 219 | 135 |
100.00%
|
100.00%
|
| Primer 15 | Ezakiella coagulans | TGCACGCCATATTCCGCA | TGATGGTGAATCGGTGCCA | 60.12 | 59.32 | 291 | 135 |
100.00%
|
100.00%
|
| Primer 16 | Ezakiella coagulans | AACGGGTCGTCTTGTCTGC | ATCGAGTGGAGCGTCATCC | 60.30 | 59.26 | 241 | 135 |
100.00%
|
100.00%
|
| Primer 17 | Ezakiella coagulans | TCAACTTGCGACCGTGCA | GCCGTACAACCTTTGACCG | 60.20 | 59.14 | 215 | 135 |
100.00%
|
100.00%
|
| Primer 18 | Ezakiella coagulans | TCGAAGACACGACGGCAA | TTTTCCGTCGGGAGAGTGC | 59.28 | 60.01 | 188 | 130 |
100.00%
|
100.00%
|
| Primer 19 | Ezakiella coagulans | TTTCGGACCGGTCACAGTG | ACGGTGCGTATGTCACAGC | 59.93 | 60.74 | 284 | 129 |
100.00%
|
100.00%
|
| Primer 20 | Ezakiella coagulans | TTTCGGACCGGTCACAGTG | GTGCGTATGTCACAGCACC | 59.93 | 59.21 | 281 | 129 |
100.00%
|
100.00%
|