| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Fibrobacter succinogenes | GGGCATGCACAATTCCGAC | TCGCGACTCCTTGCTTGT | 59.86 | 59.27 | 251 | 141 |
100.00%
|
100.00%
|
| Primer 2 | Fibrobacter succinogenes | TGCACGTGTGTTGCAGGA | AGCTTGGCCACGTCAACA | 60.12 | 59.81 | 171 | 137 |
100.00%
|
100.00%
|
| Primer 3 | Fibrobacter succinogenes | AGAATCGCACGCCACGAA | GCCTGTTCGGTCTTCGTCA | 60.05 | 60.01 | 197 | 128 |
100.00%
|
100.00%
|
| Primer 4 | Fibrobacter succinogenes | AGAATCGCACGCCACGAA | AGCTGCTTAGCCTGTTCGG | 60.05 | 60.08 | 206 | 128 |
100.00%
|
100.00%
|
| Primer 5 | Fibrobacter succinogenes | AGAATCGCACGCCACGAA | AGCCTGTTCGGTCTTCGTC | 60.05 | 59.71 | 198 | 128 |
100.00%
|
100.00%
|
| Primer 6 | Fibrobacter succinogenes | AGAATCGCACGCCACGAA | GGACTTGGCGAGTTCAGCA | 60.05 | 60.30 | 237 | 128 |
100.00%
|
100.00%
|
| Primer 7 | Fibrobacter succinogenes | AGAATCGCACGCCACGAA | TGGACTTGGCGAGTTCAGC | 60.05 | 60.30 | 238 | 128 |
100.00%
|
100.00%
|
| Primer 8 | Fibrobacter succinogenes | AGAATCGCACGCCACGAA | GCTGCTTAGCCTGTTCGGT | 60.05 | 60.38 | 205 | 128 |
100.00%
|
100.00%
|
| Primer 9 | Fibrobacter succinogenes | AGAATCGCACGCCACGAA | ATCTGGAGACCGAGCTGCT | 60.05 | 60.38 | 218 | 128 |
100.00%
|
100.00%
|
| Primer 10 | Fibrobacter succinogenes | GTGGGCATGCACAATTCCG | TCGCGACTCCTTGCTTGT | 60.15 | 59.27 | 253 | 124 |
100.00%
|
100.00%
|