| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Flaviflexus massiliensis | ACAGCGACAGACCGTGATC | TTCTCGTGCTCGCGTTGT | 59.79 | 59.97 | 221 | 80 |
100.00%
|
100.00%
|
| Primer 22 | Flaviflexus massiliensis | AACGGTCACGGCTACAGTG | TGCAGCATCAACCACGCA | 60.01 | 60.59 | 210 | 79 |
100.00%
|
100.00%
|
| Primer 23 | Flaviflexus massiliensis | CAACGGTCACGGCTACAGT | TGCAGCATCAACCACGCA | 60.01 | 60.59 | 211 | 79 |
100.00%
|
100.00%
|
| Primer 24 | Flaviflexus massiliensis | ACAACGGTCACGGCTACAG | TGCAGCATCAACCACGCA | 60.01 | 60.59 | 212 | 79 |
100.00%
|
100.00%
|
| Primer 25 | Flaviflexus massiliensis | TCACAACAGACCAGGCGAC | TGCAGCATCAACCACGCA | 59.93 | 60.59 | 293 | 79 |
100.00%
|
100.00%
|
| Primer 26 | Flaviflexus massiliensis | ATCGCGATGCACACGGAT | TCACCCAGTTCATCGTGCC | 59.89 | 60.00 | 169 | 79 |
100.00%
|
100.00%
|
| Primer 27 | Flaviflexus massiliensis | TCGAGGTCGCCAAGGAAAC | TGTCGTTCGCTTCGCAGT | 60.01 | 59.97 | 282 | 79 |
100.00%
|
100.00%
|
| Primer 28 | Flaviflexus massiliensis | CCTGCTCACCGGATGTTGT | TCAACAATGGCGAGGGCA | 60.00 | 59.57 | 274 | 79 |
100.00%
|
100.00%
|
| Primer 29 | Flaviflexus massiliensis | ACCTGCTCACCGGATGTTG | TCAACAATGGCGAGGGCA | 60.00 | 59.57 | 275 | 79 |
100.00%
|
100.00%
|
| Primer 30 | Flaviflexus massiliensis | TCACCGGATGTTGTGCAGG | TCAACAATGGCGAGGGCA | 60.30 | 59.57 | 269 | 79 |
100.00%
|
100.00%
|