| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 271 | Francisella tularensis | GGAGCTCTACACAATGGCCA | TGCCACTGAAATGCCCAGG | 59.75 | 60.61 | 295 | 68 |
100.00%
|
50.00%
|
| Primer 272 | Francisella tularensis | GCGACGTACAGGGCTGTTA | TGCGCTGAATCACCTAAAGAC | 59.79 | 58.65 | 160 | 67 |
100.00%
|
50.00%
|
| Primer 273 | Francisella tularensis | ACCGATCTTAACTCAGGGTGT | CTTGCGCTACTATCACCCCT | 58.46 | 59.25 | 240 | 66 |
100.00%
|
50.00%
|
| Primer 274 | Francisella tularensis | ACACCGATCTTAACTCAGGGT | CTTGCGCTACTATCACCCCT | 58.46 | 59.25 | 242 | 66 |
100.00%
|
50.00%
|
| Primer 275 | Francisella tularensis | ACCGATCTTAACTCAGGGTGT | TTGCGCTACTATCACCCCT | 58.46 | 58.09 | 239 | 66 |
100.00%
|
50.00%
|
| Primer 276 | Francisella tularensis | ACACCGATCTTAACTCAGGGT | TTGCGCTACTATCACCCCT | 58.46 | 58.09 | 241 | 66 |
100.00%
|
50.00%
|
| Primer 277 | Francisella tularensis | ACCCTTTGCTTGGCGAGTT | CCTGATCCGGATAATTTGCCA | 60.15 | 58.14 | 178 | 66 |
100.00%
|
50.00%
|
| Primer 278 | Francisella tularensis | ACCCTTTGCTTGGCGAGTT | ACCTGATCCGGATAATTTGCC | 60.15 | 58.14 | 179 | 66 |
100.00%
|
50.00%
|
| Primer 279 | Francisella tularensis | GCAGCAACACGCAAGCTA | TTTCGCGTGCCCAGTCTT | 59.05 | 59.89 | 199 | 66 |
100.00%
|
50.00%
|
| Primer 280 | Francisella tularensis | GCAGCAACACGCAAGCTA | TTTTCGCGTGCCCAGTCT | 59.05 | 59.89 | 200 | 66 |
100.00%
|
50.00%
|