| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Frankia casuarinae | CTGTTTGTGGGCACTGTCC | TCGATCAGACGACGACAGG | 58.97 | 58.91 | 248 | 132 |
100.00%
|
100.00%
|
| Primer 2 | Frankia casuarinae | TGGTGAGATCTGGCTGCAC | CGCCAGCGCAATTTCGAT | 59.70 | 59.60 | 208 | 131 |
100.00%
|
100.00%
|
| Primer 3 | Frankia casuarinae | AGCGATGGTGAGATCTGGC | CGCCAGCGCAATTTCGAT | 59.55 | 59.60 | 213 | 131 |
100.00%
|
100.00%
|
| Primer 4 | Frankia casuarinae | GATCTGGCTGCACAACGTG | CGCCAGCGCAATTTCGAT | 59.50 | 59.60 | 202 | 131 |
100.00%
|
100.00%
|
| Primer 5 | Frankia casuarinae | GCGGAAAAGTGGGAATGGC | ATCGCATCCCGTTCGGACT | 59.78 | 61.42 | 227 | 128 |
100.00%
|
100.00%
|
| Primer 6 | Frankia casuarinae | TGGGAATGGCAGCTGCA | ATCGCATCCCGTTCGGACT | 59.15 | 61.42 | 218 | 128 |
100.00%
|
100.00%
|
| Primer 7 | Frankia casuarinae | ACGGAGCTCTTCTTCCACC | ATCGCATCCCGTTCGGACT | 59.02 | 61.42 | 179 | 128 |
100.00%
|
100.00%
|
| Primer 8 | Frankia casuarinae | AAGTGGGAATGGCAGCTGC | ATCGCATCCCGTTCGGACT | 60.98 | 61.42 | 221 | 128 |
100.00%
|
100.00%
|
| Primer 9 | Frankia casuarinae | GGAAAAGTGGGAATGGCAGC | ATCGCATCCCGTTCGGACT | 59.75 | 61.42 | 225 | 128 |
100.00%
|
100.00%
|
| Primer 10 | Frankia casuarinae | TGTTTCGCACCGTGATGGA | TGCTTCTCAACGAGCGCA | 59.93 | 59.97 | 229 | 128 |
100.00%
|
100.00%
|