| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1251 | Frankia casuarinae | TCTTGTCCGAACTCGCGA | GCGGTCAGACAGAACAGGA | 58.65 | 59.33 | 205 | 39 |
100.00%
|
100.00%
|
| Primer 1252 | Frankia casuarinae | TGGAGACGTTCCCGATCGA | GCCACCAGATCGTCACGTA | 60.38 | 59.49 | 232 | 39 |
100.00%
|
100.00%
|
| Primer 1253 | Frankia casuarinae | TCAGCTGGAGATGCGCAT | TCTTCAGGTCCTCCAGCGA | 59.09 | 59.92 | 194 | 39 |
100.00%
|
100.00%
|
| Primer 1254 | Frankia casuarinae | ATGACCGACTTCCCGACGA | TACTCGTCCACCTCGCTCA | 60.68 | 60.00 | 196 | 39 |
100.00%
|
100.00%
|
| Primer 1255 | Frankia casuarinae | AGAATCGGGACATGGCCA | CGTTGGTCAGCCGAATCGA | 58.26 | 60.45 | 168 | 39 |
100.00%
|
100.00%
|
| Primer 1256 | Frankia casuarinae | GGATCTTTCGCCTGACCGA | ATCAGGGAGCGGTACACCT | 59.48 | 60.00 | 202 | 39 |
100.00%
|
100.00%
|
| Primer 1257 | Frankia casuarinae | GAACTGGCAGCAGATCCGA | AGCTCGCGTATCCTGCTT | 59.78 | 58.79 | 156 | 39 |
100.00%
|
100.00%
|
| Primer 1258 | Frankia casuarinae | ATGAAGTCCCGTGACGAGC | TTCGGCAGGTACTCCTCGA | 59.78 | 60.00 | 239 | 39 |
100.00%
|
100.00%
|
| Primer 1259 | Frankia casuarinae | AGCAGGAGGATCTCACCGA | TCGCCACGTTCGACAGTT | 59.69 | 59.59 | 237 | 39 |
100.00%
|
100.00%
|
| Primer 1260 | Frankia casuarinae | ATCTGACCGATCCCAGCCT | ACCGGACCACCAGCAGATA | 60.07 | 60.31 | 171 | 39 |
100.00%
|
100.00%
|