| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 61 | Fusobacterium necrophorum | GAAGCACAGAGAGCGGAAGA | TGCAGTCGAACCAAGGCA | 59.76 | 59.49 | 236 | 125 |
100.00%
|
50.00%
|
| Primer 62 | Fusobacterium necrophorum | AAGCACAGAGAGCGGAAGA | TGCAGTCGAACCAAGGCA | 58.64 | 59.49 | 235 | 125 |
100.00%
|
50.00%
|
| Primer 63 | Fusobacterium necrophorum | GCCAGAGTTGCCAGAGCTT | TTCCGGTCTGGGAGGTCTT | 60.30 | 59.84 | 222 | 125 |
100.00%
|
50.00%
|
| Primer 64 | Fusobacterium necrophorum | TGCCATTACCGTGGGAGTG | TACCTTACCCCACCTGCCA | 59.70 | 59.84 | 232 | 124 |
100.00%
|
50.00%
|
| Primer 65 | Fusobacterium necrophorum | TGCAATGCAGCATCCGGA | ACGCCATTAAAGTTTGTGCCA | 60.05 | 59.32 | 260 | 124 |
100.00%
|
50.00%
|
| Primer 66 | Fusobacterium necrophorum | GCATTTGTAGCAGGTGCCG | TTCACTTCCGGTTGCCCA | 59.86 | 59.08 | 227 | 124 |
100.00%
|
50.00%
|
| Primer 67 | Fusobacterium necrophorum | GCATTTGTAGCAGGTGCCG | TCACTTCCGGTTGCCCAAA | 59.86 | 59.77 | 226 | 124 |
100.00%
|
50.00%
|
| Primer 68 | Fusobacterium necrophorum | GCATTTGTAGCAGGTGCCG | ATTCACTTCCGGTTGCCCA | 59.86 | 59.54 | 228 | 124 |
100.00%
|
50.00%
|
| Primer 69 | Fusobacterium necrophorum | GCATTTGTAGCAGGTGCCG | TCTTTTGCCCTCCCTGGTC | 59.86 | 59.23 | 299 | 124 |
100.00%
|
50.00%
|
| Primer 70 | Fusobacterium necrophorum | GCATTTGTAGCAGGTGCCG | CACTTCCGGTTGCCCAAA | 59.86 | 58.15 | 225 | 124 |
100.00%
|
50.00%
|