| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Gracilibacillus massiliensis | TGCAGCTGGAGCACTTGT | ACCGGATGAAAGCTGCTGT | 59.49 | 59.62 | 181 | 148 |
100.00%
|
100.00%
|
| Primer 12 | Gracilibacillus massiliensis | AGAACCTGTTGCCGAGTGG | TGGCTGCTAAATGACCTCCAG | 59.93 | 60.07 | 206 | 148 |
100.00%
|
100.00%
|
| Primer 13 | Gracilibacillus massiliensis | AGAGCGATCCTATGTGGCAC | ACGTCCAATCGCGTTTGC | 59.61 | 59.45 | 292 | 146 |
100.00%
|
100.00%
|
| Primer 14 | Gracilibacillus massiliensis | TGCGCTAAAAGCTGGTGC | ATAGGCTACCGCAACATGTG | 59.05 | 58.05 | 187 | 146 |
100.00%
|
100.00%
|
| Primer 15 | Gracilibacillus massiliensis | GGCCTAGGATTGAGAGGTGG | TGTAGGCAACAAACTGACTGG | 59.24 | 58.43 | 177 | 145 |
100.00%
|
100.00%
|
| Primer 16 | Gracilibacillus massiliensis | TGTCGAAAGCGGTGCAGT | TCGTCACCTTCCCGTAACAG | 59.89 | 59.40 | 189 | 143 |
100.00%
|
100.00%
|
| Primer 17 | Gracilibacillus massiliensis | TGTCGAAAGCGGTGCAGT | TCTCGTCACCTTCCCGTAAC | 59.89 | 59.12 | 191 | 143 |
100.00%
|
100.00%
|
| Primer 18 | Gracilibacillus massiliensis | AGAACCTGTTGCCGAGTGG | GCTGCTAAATGACCTCCAGC | 59.93 | 58.98 | 204 | 143 |
100.00%
|
100.00%
|
| Primer 19 | Gracilibacillus massiliensis | AGTAGGCCTCGTAGTAGGTGG | ATTTGATCGCCTAACTGCAGT | 60.13 | 58.01 | 222 | 143 |
100.00%
|
100.00%
|
| Primer 20 | Gracilibacillus massiliensis | CGGTAGCAAGAAAATGGGTGC | ATTTGATCGCCTAACTGCAGT | 60.14 | 58.01 | 172 | 143 |
100.00%
|
100.00%
|