| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Histophilus somni | CGGCAAAAGTCCGGAGCAT | GCACCCCACAATTCTTGGC | 61.04 | 59.71 | 192 | 143 |
100.00%
|
100.00%
|
| Primer 2 | Histophilus somni | ACGTCAACAAGGTGCGAGT | ACCAGATCGAAACGGCAGT | 59.86 | 59.33 | 291 | 143 |
100.00%
|
100.00%
|
| Primer 3 | Histophilus somni | ACAGAGGCTGAGTTGGAGC | CCGTGGCTAATAAATGCGGTG | 59.32 | 60.00 | 205 | 140 |
100.00%
|
100.00%
|
| Primer 4 | Histophilus somni | TGGTGTCATTGCTCGTGC | TGCGGTCGTAGCATTGCA | 58.35 | 60.05 | 216 | 138 |
100.00%
|
100.00%
|
| Primer 5 | Histophilus somni | TTGGTGTCATTGCTCGTGC | TGCGGTCGTAGCATTGCA | 59.05 | 60.05 | 217 | 138 |
100.00%
|
100.00%
|
| Primer 6 | Histophilus somni | CTTGGTGTCATTGCTCGTGC | TGCGGTCGTAGCATTGCA | 60.11 | 60.05 | 218 | 138 |
100.00%
|
100.00%
|
| Primer 7 | Histophilus somni | TTGCCGAATTGGACCGCA | ACGGTCCAACAGGAACACC | 60.28 | 59.85 | 184 | 138 |
100.00%
|
100.00%
|
| Primer 8 | Histophilus somni | TTGCCGAATTGGACCGCA | CCGCTGCAATTAACGGTCC | 60.28 | 59.57 | 196 | 138 |
100.00%
|
100.00%
|
| Primer 9 | Histophilus somni | TGCGGATTCTCGTGGTGT | TGAACAAACGTGCAGCCG | 58.94 | 59.29 | 209 | 135 |
100.00%
|
100.00%
|
| Primer 10 | Histophilus somni | TGGCATCAAACTTCAGGGTCA | GCTAATGCTTCCGCTTGCA | 59.86 | 59.20 | 189 | 132 |
100.00%
|
100.00%
|