| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Holdemania filiformis | TCGACGTTTCTGCGGTGT | TCAGCGAGTGTTCCACGAC | 59.59 | 60.01 | 256 | 141 |
100.00%
|
50.00%
|
| Primer 2 | Holdemania filiformis | CGTAATCCGGATGGTCGAGG | TTCCGGTTGGCCATGCAT | 60.04 | 59.96 | 151 | 118 |
100.00%
|
50.00%
|
| Primer 3 | Holdemania filiformis | TCGACGTTTCTGCGGTGT | CCAGCACGGTTAAATGCGG | 59.59 | 59.86 | 166 | 115 |
100.00%
|
50.00%
|
| Primer 4 | Holdemania filiformis | TCCGGAAAAGGTGGTGTGG | AAAGCGCATGGAAACGGC | 59.85 | 59.74 | 184 | 112 |
100.00%
|
50.00%
|
| Primer 5 | Holdemania filiformis | ATCACGGAAGCCAGCGAT | TGCACTGTGTCCGCAGAT | 59.10 | 59.26 | 286 | 110 |
100.00%
|
50.00%
|
| Primer 6 | Holdemania filiformis | ACGACGAAGAAGACGCTGG | TGCACTGTGTCCGCAGAT | 60.08 | 59.26 | 169 | 110 |
100.00%
|
50.00%
|
| Primer 7 | Holdemania filiformis | GATCACGGAAGCCAGCGAT | TGCACTGTGTCCGCAGAT | 60.23 | 59.26 | 287 | 110 |
100.00%
|
50.00%
|
| Primer 8 | Holdemania filiformis | GAAAGTTTGGCGAGCGACC | AACCTCTGCTTCCTCGCAC | 59.79 | 60.00 | 216 | 107 |
100.00%
|
50.00%
|
| Primer 9 | Holdemania filiformis | GCCAGCTGCGCAATGAAA | TACTGTACCGTTGCGCACC | 59.74 | 60.37 | 275 | 106 |
100.00%
|
50.00%
|
| Primer 10 | Holdemania filiformis | TGCGGTCGCTGTTTTCCA | AAGTTGCCGTCGACGACT | 60.20 | 59.27 | 234 | 104 |
100.00%
|
50.00%
|