| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 101 | Holdemania filiformis | ATCGAACCGCTGCATGGT | CCGTCGCGACAATCGTTT | 59.73 | 58.85 | 229 | 89 |
100.00%
|
50.00%
|
| Primer 102 | Holdemania filiformis | AATGCGTGCGGATCGTCT | ATCGCTTCGTCTTCCGCA | 59.82 | 59.43 | 163 | 89 |
100.00%
|
50.00%
|
| Primer 103 | Holdemania filiformis | AGGTGCGTCCGCTGATTT | TCCGGACTTTGTCGCCAT | 59.65 | 58.93 | 264 | 89 |
100.00%
|
50.00%
|
| Primer 104 | Holdemania filiformis | AGGTGCGTCCGCTGATTT | ATCCGGACTTTGTCGCCA | 59.65 | 58.93 | 265 | 89 |
100.00%
|
50.00%
|
| Primer 105 | Holdemania filiformis | AGGTGCGTCCGCTGATTT | TCCGGACTTTGTCGCCATC | 59.65 | 60.08 | 264 | 89 |
100.00%
|
50.00%
|
| Primer 106 | Holdemania filiformis | AGGTGCGTCCGCTGATTT | GATCCGGACTTTGTCGCCA | 59.65 | 60.08 | 266 | 89 |
100.00%
|
50.00%
|
| Primer 107 | Holdemania filiformis | AGGTGCGTCCGCTGATTT | CGACGACGATCCGGACTTT | 59.65 | 59.86 | 273 | 89 |
100.00%
|
50.00%
|
| Primer 108 | Holdemania filiformis | AGGTGCGTCCGCTGATTT | GATCCACGTCCATTCCGCT | 59.65 | 59.86 | 165 | 89 |
100.00%
|
50.00%
|
| Primer 109 | Holdemania filiformis | AGGTGCGTCCGCTGATTT | AGATCCACGTCCATTCCGC | 59.65 | 59.86 | 166 | 89 |
100.00%
|
50.00%
|
| Primer 110 | Holdemania filiformis | AGGTGCGTCCGCTGATTT | ATCCACGTCCATTCCGCTG | 59.65 | 60.15 | 164 | 89 |
100.00%
|
50.00%
|