| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1251 | Holdemania filiformis | ACGCAAGCCAATCCGGAA | GCAAATCTCGCACGGCTT | 59.97 | 59.13 | 283 | 40 |
100.00%
|
50.00%
|
| Primer 1252 | Holdemania filiformis | CAGCTGGAGATGCGTGTGA | CGCCACATTCTGCGCAAA | 60.08 | 59.75 | 156 | 40 |
100.00%
|
50.00%
|
| Primer 1253 | Holdemania filiformis | AGCGGTTCTGCATGGCTT | AGGAACCGCACATTTTGGC | 59.97 | 59.33 | 263 | 40 |
100.00%
|
50.00%
|
| Primer 1254 | Holdemania filiformis | AGCGGTTCTGCATGGCTT | AAGGAACCGCACATTTTGGC | 59.97 | 59.97 | 264 | 40 |
100.00%
|
50.00%
|
| Primer 1255 | Holdemania filiformis | AGCTGAATTCCGTGCCCAA | AATGCTCAGGCGTCGCTT | 59.93 | 60.05 | 260 | 40 |
100.00%
|
50.00%
|
| Primer 1256 | Holdemania filiformis | TTGAAATCGTGCGCAGGC | CCGTCAACAAGCTTTCCGC | 59.44 | 60.08 | 267 | 40 |
100.00%
|
50.00%
|
| Primer 1257 | Holdemania filiformis | CGCTCTGCAATTTCGCGA | ACCGCATTGCCTGGTTCA | 59.22 | 59.88 | 290 | 40 |
100.00%
|
50.00%
|
| Primer 1258 | Holdemania filiformis | TTACGGCATGCTTCGCGA | TTCCGGAGCACTGCGTTT | 60.13 | 59.89 | 247 | 40 |
100.00%
|
50.00%
|
| Primer 1259 | Holdemania filiformis | TTACGGCATGCTTCGCGA | TTTCCGGAGCACTGCGTT | 60.13 | 59.89 | 248 | 40 |
100.00%
|
50.00%
|
| Primer 1260 | Holdemania filiformis | TCGGCAGGCTCATTCGTT | TTCTTGTCTTTGCCCATTCGA | 59.34 | 58.14 | 221 | 40 |
100.00%
|
50.00%
|