| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Holdemania filiformis | TCGGGTGAATGGCAACTGG | ACAGGCAGGTATAGCGCAG | 60.30 | 59.56 | 155 | 104 |
100.00%
|
50.00%
|
| Primer 12 | Holdemania filiformis | TGATGAACCGCTGCTGCA | AGCAGGAACCTTGAACCCG | 59.97 | 59.93 | 230 | 104 |
100.00%
|
50.00%
|
| Primer 13 | Holdemania filiformis | TGCGATCTTGGGCAGCAT | ATAGCCATCCTTGCCGACC | 59.73 | 59.55 | 202 | 103 |
100.00%
|
50.00%
|
| Primer 14 | Holdemania filiformis | GCGTTCAGGAAAGCGGTGT | CCGCTGACAAAAGACGACTG | 61.25 | 59.49 | 173 | 102 |
100.00%
|
50.00%
|
| Primer 15 | Holdemania filiformis | ATCCGATGCTCCATGCCTG | AAAGCTGACCGGTCGGAAG | 59.93 | 60.00 | 215 | 102 |
100.00%
|
50.00%
|
| Primer 16 | Holdemania filiformis | AGCTGGGTTTCGATCGACG | TGCACTGTGTCCGCAGAT | 60.15 | 59.26 | 202 | 101 |
100.00%
|
50.00%
|
| Primer 17 | Holdemania filiformis | CGGCTGTATGAACGCGTTG | TGCGAGCCGATGCATTCA | 59.94 | 60.13 | 157 | 101 |
100.00%
|
50.00%
|
| Primer 18 | Holdemania filiformis | TGCGACAGGAGGTGGAAAG | TGCGAGCCGATGCATTCA | 59.63 | 60.13 | 186 | 101 |
100.00%
|
50.00%
|
| Primer 19 | Holdemania filiformis | CTGCGACAGGAGGTGGAAA | TGCGAGCCGATGCATTCA | 59.63 | 60.13 | 187 | 101 |
100.00%
|
50.00%
|
| Primer 20 | Holdemania filiformis | TTGCTGGGGTGTGCTTCA | TGAGCGTTGGGAAGCAGT | 59.40 | 59.17 | 284 | 101 |
100.00%
|
50.00%
|