| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Holdemania filiformis | AGGCGTCAAGGGCTTTGT | AATCGTCTCGCAGCTGCA | 59.48 | 59.74 | 190 | 100 |
100.00%
|
50.00%
|
| Primer 22 | Holdemania filiformis | CGAAACCCACAACCGAGGA | TAATCCTGCAGCTGCGCA | 59.93 | 59.73 | 160 | 100 |
100.00%
|
50.00%
|
| Primer 23 | Holdemania filiformis | ACGAAACCCACAACCGAGG | TAATCCTGCAGCTGCGCA | 60.23 | 59.73 | 161 | 100 |
100.00%
|
50.00%
|
| Primer 24 | Holdemania filiformis | TCTGTGTCTGGTTGGCTGC | TAATCCTGCAGCTGCGCA | 60.23 | 59.73 | 183 | 100 |
100.00%
|
50.00%
|
| Primer 25 | Holdemania filiformis | GTTGGCTGCCAGACGAAAC | TAATCCTGCAGCTGCGCA | 59.72 | 59.73 | 173 | 100 |
100.00%
|
50.00%
|
| Primer 26 | Holdemania filiformis | CGGCTGCGCTTTCAACAA | TGCGACCTCGATTTCACCC | 59.67 | 60.08 | 235 | 99 |
100.00%
|
50.00%
|
| Primer 27 | Holdemania filiformis | CGGCTGCGCTTTCAACAA | TCCGATTGCCGTGATCAGG | 59.67 | 59.86 | 271 | 99 |
100.00%
|
50.00%
|
| Primer 28 | Holdemania filiformis | CGGCTGCGCTTTCAACAA | CGACTCCGATTGCCGTGAT | 59.67 | 60.23 | 275 | 99 |
100.00%
|
50.00%
|
| Primer 29 | Holdemania filiformis | CGGCTGCGCTTTCAACAA | ACCTCGATTTCACCCCAGC | 59.67 | 59.70 | 231 | 99 |
100.00%
|
50.00%
|
| Primer 30 | Holdemania filiformis | GCCAGCTGCGCAATGAAA | TCCAGACAATGATCGACGGC | 59.74 | 60.18 | 172 | 99 |
100.00%
|
50.00%
|