| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 71 | Holdemania filiformis | ACCGATCGAACGACGCTT | CGCTGCCCTGAAACGAAA | 59.44 | 58.66 | 225 | 94 |
100.00%
|
50.00%
|
| Primer 72 | Holdemania filiformis | GCGTTTTGTTTCTGGTTTGGC | GCCGATAGCAGTCACGATCA | 59.67 | 59.97 | 156 | 94 |
100.00%
|
50.00%
|
| Primer 73 | Holdemania filiformis | ACGGAAGCCAGCGATATCG | TGCACTGTGTCCGCAGAT | 60.01 | 59.26 | 283 | 93 |
100.00%
|
50.00%
|
| Primer 74 | Holdemania filiformis | GCGTTCAGGAAAGCGGTGT | TCCGCTGACAAAAGACGACT | 61.25 | 59.61 | 174 | 92 |
100.00%
|
50.00%
|
| Primer 75 | Holdemania filiformis | TGCCAGACGAAACCCACAA | TAATCCTGCAGCTGCGCA | 59.78 | 59.73 | 167 | 92 |
100.00%
|
50.00%
|
| Primer 76 | Holdemania filiformis | TTCGGGATGCTGCAGAAGG | AGCGCTTTGGCTACGACT | 60.08 | 59.34 | 205 | 92 |
100.00%
|
50.00%
|
| Primer 77 | Holdemania filiformis | TGATGCTGCAGATGCGCT | TGTGCATGACCGCAAACC | 60.12 | 58.96 | 189 | 90 |
100.00%
|
50.00%
|
| Primer 78 | Holdemania filiformis | GGTGTGCAGGGCAACAATG | AGCGCACCGTGTTAAGCT | 60.01 | 59.97 | 187 | 90 |
100.00%
|
50.00%
|
| Primer 79 | Holdemania filiformis | GCGAACCGGAAATCTGGGA | AGCGCACCGTGTTAAGCT | 60.08 | 59.97 | 234 | 90 |
100.00%
|
50.00%
|
| Primer 80 | Holdemania filiformis | GTGCAGGGCAACAATGACG | AGCGCACCGTGTTAAGCT | 60.08 | 59.97 | 184 | 90 |
100.00%
|
50.00%
|