| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 801 | Holdemania filiformis | AGCGGTGCGATTGATGGA | TGCGTGCCGATTCCATGA | 59.42 | 59.73 | 165 | 55 |
100.00%
|
50.00%
|
| Primer 802 | Holdemania filiformis | AGAACTGCGCTCCGTTGT | GATGATTGCCATCGGTGCG | 59.58 | 59.72 | 238 | 55 |
100.00%
|
50.00%
|
| Primer 803 | Holdemania filiformis | TGTTCGCAGCGTTTGGGA | ATCAGACCCAAAGCCGTGG | 60.20 | 60.00 | 268 | 55 |
100.00%
|
50.00%
|
| Primer 804 | Holdemania filiformis | GGACGATCGCCTGCTTCAT | TGCCTGCGCACGATTTCA | 60.23 | 60.67 | 192 | 55 |
100.00%
|
50.00%
|
| Primer 805 | Holdemania filiformis | ATGTCACGACGATCGCGA | GCACAGCACGTTGCCAAA | 59.21 | 59.90 | 269 | 55 |
100.00%
|
50.00%
|
| Primer 806 | Holdemania filiformis | AGCGACTGCACGGATTCA | ACCGTGGCGTCAACATGA | 59.34 | 59.58 | 189 | 55 |
100.00%
|
50.00%
|
| Primer 807 | Holdemania filiformis | AGCGACTGCACGGATTCA | TCACCGTGGCGTCAACAT | 59.34 | 59.58 | 191 | 55 |
100.00%
|
50.00%
|
| Primer 808 | Holdemania filiformis | CCCCTGTTAAAGCGCTGGA | TTACCAGAGCGTCGCTGT | 60.00 | 58.64 | 156 | 55 |
100.00%
|
50.00%
|
| Primer 809 | Holdemania filiformis | ACTGATTGCCTGCGTCGA | ACGCCTTAAAGCTGCACG | 59.34 | 58.75 | 284 | 55 |
100.00%
|
50.00%
|
| Primer 810 | Holdemania filiformis | AATTGCCAAGGCGATGCG | ACTTCACGGCAGTCGCTT | 59.51 | 59.58 | 222 | 55 |
100.00%
|
50.00%
|